The Tongling metallogenic district is one of the important copper-gold-polymetallic ore belts in East China. The Qingshuitang copper deposit is a typical skarn-type copper deposit in the district, which is located in the Fenghuangshan orefield. Re-Os dating of molybdenite from the Qingshuitang copper deposit has been carried out to constrain the age of mineralization. The molybdenite samples have Re-Os model ages range from 140.9±1.1 Ma to 141.4±1.1 Ma, with an average age of 141.3±0.7 Ma. The Re-Os isochron age of 142.0±1.7 Ma is consistent with the zircon U-Pb age (141.3±1.3 Ma) of the Xinwuli granodiorite. The dating results indicate that the Qingshuitang copper deposit is genetically related to the Early Cretaceous Xinwuli granodiorite. The Re contents of molybdenite range from 2.23×10-6 to 4.94×10-6, indicative of a crustal source. Formation of the Qingshuitang copper deposit is consistent with the peak period (135 to 145 Ma) of copper-gold polymetallic mineralization in the Tongling metallogenic district. Therefore, the Qingshuitang copper deposit was formed under a tectonic transition environment from compression to extension. © 2020, Science Press. All right reserved.