Experimental study on influencing factors of microbial gas production performance

To study the influences of multiple factors on the gas production performance of microorganisms, surfactants were selected to improve the stability of bubbles, and a control method for desaturation was obtained. The effects of temperature, pH value, microbial concentration, nitrogen source mass concentration, surfactant type, and dosage on the gas production performance of Pseudomonas stutzen were analyzed by a six-factor five-level orthogonal experiment. Experimental results show that when the temperature is between 4 °C and 30 °C, the gas production performance increases with the increase of temperatures, and the gas production rate at 30 °C is 5 times that at 4 °C. The pH value has a greater effect on the stagnation time, and the stagnation time in the alkaline environment is obvious lower than that in the acidic environment, the stagnation time at pH = 9 is 53 h less than that at pH = 5. The gas production performance first increases and then weakens with the increase of the microbial concentration, the optimal microbial concentration is optical density OD600=0.10. The gas production at the nitrogen source mass concentration of 3. 0 g/L is 5. 3 times that of 1.0 g/L, and the gas production has a linear relationship with the carbon-nitrogen mass ratio. The stagnation time, the gas production time and the gas production rate are mostly affected by temperature; the gas production is determined by the mass concentration of the nitrogen source. © 2022 Southeast University. All rights reserved.