Isolation, Purification and Applications of Antioxidant Peptides from Whey Protein Hydrolysis

被引：0
|
作者：
Wang H. [1 ]
Liu Y. [1 ]
Zhang J. [1 ]
Zhang H. [1 ]
Sun T. [1 ]
Yao G. [1 ]
机构：
[1] Inner Mongolia Agricultural University, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineer
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 04期
关键词：
antioxidant peptides; Bifidobacterium animalis subsp. lactis; freeze-drying; isolation and purification; whey protein;
D O I：
10.16429/j.1009-7848.2023.04.004
中图分类号：
学科分类号：
摘要：
Objective: A peptide with antioxidant activity was screened from whey protein hydrolysate and used to protect Probio-M8 during the freeze-drying process. The whey proteins were hydrolyzed by alkaline protease and separated by ultrafiltration and three different molecular weight peptide samples were obtained, k1 (>8 ku), k2 (3-8 ku), and k3 (<3 ku). The peptides were then purified and identified by strong cation exchange chromatography, reversed-phase high-performance liquid chromatography (RP-HPLC), and liquid chromatography-mass spectrometry (LC-MS). 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging, resistance to superoxide anion radicals, and absorption of oxidative radicals were used as assays to assess antioxidant activity and were screened. Results: Samples 3-2 and 4-6 showed high antioxidant activity. Five and six antioxidant peptide sequences were identified for samples 3-2 and 4-6, respectively. Sample 3-2 was able to protect B. lactis Probio-M8 in vacuum freeze-drying, increasing the survival rate of the strain to (88.31±0.02)% and also reducing intracellular reactive oxygen species levels and protecting cellular enzyme activity. Conclusion: Sample 3-2 was effective in reducing cell damage caused by the vacuum freeze-drying process of B. lactis Probio-M8. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：35 / 47
页数：12
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