Effect of Tea Tree Oil on Methicillin-resistant Staphylococcus aureus Biofilm

被引：0
|
作者：
Cheng F. [1 ]
Liu Y. [1 ]
Yang Z. [1 ]
Mo Y. [1 ]
Shang R. [1 ]
Hao B. [1 ]
Wang X. [1 ]
Liang J. [1 ]
机构：
[1] Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Scie
来源：
Science and Technology of Food Industry | 2021年 / 42卷 / 14期
关键词：
biofilm; CLSM; eDNA; PIA; semi-quantitative crystal violet experiments; tea tree oil;
D O I：
10.13386/j.issn1002-0306.2020110063
中图分类号：
学科分类号：
摘要：
The effects of tea tree oil on the biofilm of Methicillin-resistant Staphylococcus aureus (MRSA) and its mechanism of action were studied in this paper . Firstly, the MIC values of tea tree oil against MRSA and 12 clinically isolated strains were determined to be 0.08%~0.32%. It was found that 0.16% of tea tree oil had an inhibitory effect on the formation of MRSA biofilms. Tea tree oil at 0.32% could completely remove mature biofilms of MRSA while 0.16% could significantly reduce the mature biofilms by semi-quantitative crystal violet experiments and CLSM. Preliminary exploration of the biofilm mechanism found that tea tree oil could inhibit the secretion of polysaccharide intercellular adhesion (PIA) and extracellular DNA (eDNA), and the expression level of agr, ica, cid and sar during the formation of MRSA biofilm. Therefore, tea tree essential oil would have a strong anti-biofilm effect, and could inhibite the synthesis of PIA, the secretion of eDNA and the expression of genes related to biofilm formation. © 2021 Chinese Medical Journals Publishing House Co.Ltd
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页码：107 / 112
页数：5
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