Sesame oils are subjected to different extraction methods including compression, aqueous extraction, and refining that includes degumming, alkali refining, bleaching, and deodorizing. This research investigated the fatty acid distribution and composition of triacylglycerols (TAGs) in the sesame oils and the acid value (AV), peroxides value (PV), sesamin, sesamolin, and total tocopherols. The results showed that sesame oil obtained by aqueous extraction had a higher content of sesamin, sesamolin, and tocopherols but lower free fatty acids (FFA) and peroxides. Aqueous extraction of sesame oil had the highest percentage of polyunsaturated fatty acids (PUFA) and lowest content of saturated fatty acids (SFA). Refining sesame oil had the lowest and the highest contents of PUFA and SFA, respectively. After different extraction methods and refining steps, the fatty acids' positional distribution's main specifications were retained. The composition of TAGs showed no significant difference between the compression and aqueous extraction of sesame oil, while the TAGs composition of refining sesame oil had a small but significant difference in comparison to the compression and aqueous extraction of sesame oil (P © (2024), (Bio Tech System). All Rights Reserved.