Enhanced exosome capture in urine using aptamer-modified temperature-responsive polymer for sensitive early detection of bladder cancer

被引：0

作者：

Liu, Min ^{[1
,2
]}

Yi, Kezhen ^{[1
,2
]}

Zhang, Yaran ^{[3
]}

Long, Fei ^{[1
,2
]}

Hu, Xin ^{[1
,2
]}

Jia, Gaihua ^{[1
,2
]}

Xiao, Ting ^{[1
,2
]}

Xu, Xianqun ^{[1
]}

Duan, Yongwei ^{[1
]}

Shi, Hongjie ^{[4
]}

Yao, Zhipeng ^{[5
]}

Liu, Tongzu ^{[6
]}

He, Zhike ^{[3
,7
]}

Liu, Zheng ^{[5
]}

Wang, Fubing ^{[1
,2
,8
]}

Wang, Xinghuan ^{[6
,8
]}

机构：

[1] Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan,430071, China

[2] Center for Single-Cell Omics and Tumor Liquid Biopsy, Zhongnan Hospital of Wuhan University, Wuhan,430071, China

[3] College of Chemistry and Molecular Sciences, Wuhan University, Wuhan,430072, China

[4] Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan,430071, China

[5] Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030, China

[6] Department of Urology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan,430071, China

[7] Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Province Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan,430071, China

[8] Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan,430071, China

来源：

Chemical Engineering Journal | 2024年 / 489卷

关键词：

Current methods for bladder cancer (BCa) detection are often invasive and yield unsatisfactory diagnostic performance; especially for low-grade and early-stage tumors. Exosome biomarkers in urine offer excellent potential for improving the non-invasive early diagnosis of BCa. However; the lack of an efficient exosome isolation strategy poses a challenge for clinical application. This study presents a strategy for homogeneous exosome capture to enhance enrichment efficiency. We decorated exosome-specific aptamers onto a temperature-responsive polymer. Subsequently; leveraging the thermoresponsive properties of the polymer; which can transition between homogeneous and heterogeneous states at different temperatures; we achieved efficient homogeneous capture and rapid heterogeneous separation of urinary exosomes. The procedure; conducted at a relatively low temperature; enables rapid exosome isolation (within 5 min) without ultracentrifugation; facilitating clinical application. The developed exosome enrichment method using the functionalized temperature-responsive polymer offers significant advantages: an efficient; rapid; low-cost method that is widely applicable. Exosomes from urine samples; enriched using a functionalized temperature-responsive polymer; established a diagnostic model; exhibiting significant improved sensitivity over fluorescence in situ hybridization (FISH); particularly for early-stage (88.7% vs. 49.1%) and low-grade BCa (82.6% vs. 26.1%). This non-invasive; reliable BCa detection method holds promising potential for clinical use. © 2024 Elsevier B.V;

D O I：

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学科分类号：

摘要：

Journal article (JA)

引用

共 39 条

[1] Enhanced exosome capture in urine using aptamer-modified temperature-responsive polymer for sensitive early detection of bladder cancer
Liu, Min
Yi, Kezhen
Zhang, Yaran
Long, Fei
Hu, Xin
Jia, Gaihua
Xiao, Ting
Xu, Xianqun
Duan, Yongwei
Shi, Hongjie
Yao, Zhipeng
Liu, Tongzu
He, Zhike
Liu, Zheng
Wang, Fubing
Wang, Xinghuan
CHEMICAL ENGINEERING JOURNAL, 2024, 489
[2] Highly sensitive detection for cocaine using an aptamer-modified molybdenum disulfide/gold nanoparticle microarray
Gao, Li
Deng, Zebin
Lin, Yuanwei
Sulemana, Husseini
Shi, Haixia
Yu, Chaosheng
Chen, Shuaijun
NEW JOURNAL OF CHEMISTRY, 2020, 44 (31) : 13466 - 13471
[3] Enhanced cellular uptake and gene silencing activity of siRNA using temperature-responsive polymer-modified liposome
Wang, Jian
Ayano, Ed
Maitani, Yoshie
Kanazawa, Hideko
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2017, 523 (01) : 217 - 228
[4] A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe
Aihui Liang
Lianping Zhou
Zhiliang Jiang
Plasmonics, 2011, 6 : 387 - 392
[5] A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe
Liang, Aihui
Zhou, Lianping
Jiang, Zhiliang
PLASMONICS, 2011, 6 (02) : 387 - 392
[6] Highly Sensitive Resonance Scattering Detection of DNA Hybridization Using Aptamer-Modified Gold Nanopaticle as Catalyst
Huilin Tao
Lili Wei
Aihui Liang
Jianfu Li
Zhiliang Jiang
Hesheng Jiang
Plasmonics, 2010, 5 : 189 - 198
[7] Highly Sensitive Resonance Scattering Detection of DNA Hybridization Using Aptamer-Modified Gold Nanopaticle as Catalyst
Tao, Huilin
Wei, Lili
Liang, Aihui
Li, Jianfu
Jiang, Zhiliang
Jiang, Hesheng
PLASMONICS, 2010, 5 (02) : 189 - 198
[8] Aqueous chromatography system using temperature-responsive polymer-modified stationary phases
Ayano, E
Kanazawa, H
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (06) : 738 - 749
[9] Temperature-Responsive Chromatographic System Using N-Isopropylacrylamide Polymer-Modified Columns
Dong, Wei
Fan, Xu
Cao, Guang Qun
Zhu, Wen Jia
MATERIAL SCIENCE, CIVIL ENGINEERING AND ARCHITECTURE SCIENCE, MECHANICAL ENGINEERING AND MANUFACTURING TECHNOLOGY II, 2014, 651-653 : 124 - 127
[10] Temperature-Responsive Chromatography Using a Functional Polymer Modified Stationary Phase with Molecular Recognition Sites
Sakata, Kazuki
Ohkubo, Kohei
Hiruta, Yuki
Ayano, Eri
Kanazawa, Hideko
KOBUNSHI RONBUNSHU, 2014, 71 (07) : 293 - 301

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