Effects of Total Glycosides of Cistanche deserticola on Proliferation, Apoptosis and Expression of Wnt/β-Catenin Signaling Pathway Related Protein of HepG2 Cells

被引：0
|
作者：
Feng D. [1 ,2 ]
Wang J. [2 ]
Jiang Y. [3 ]
Zhou S. [1 ]
Duan H. [1 ]
Guo Y. [1 ]
Zhao J. [1 ]
Yan W. [1 ]
机构：
[1] Beijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing
[2] Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing
[3] Inner Mongolia Sankou Biotechnology Co., Ltd., Ordos
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 20期
关键词：
anti-hepatoma; Cistanche deserticola Y.C. Ma; HepG2; cell; total glycosides of Cistanche deserticola; Wnt/βcatenin signal pathway;
D O I：
10.13386/j.issn1002-0306.2022100297
中图分类号：
学科分类号：
摘要：
To explore the inhibitory effect of total glycosides of Cistanche deserticola (TG) on HepG2 cells and its mechanism. In this paper, different concentrations (0, 3.5, 10.5, 21, 31.5, 42 μg/mL) TG were treated 24 h on HepG2 liver cancer cells, and the viability of HepG2 cells was detected using CCK8 assay. Hoechst 33342/PI double staining method and Annexin V-FITC/PI were used to detect HepG2 cells apoptosis. The phenomenon of cell migration was detected by cell migration assay. Meanwhile, cell cycle progression changes were detected by flow cytometry. And the expression of αfetoprotein (AFP), β-catenin, Dishevelled (Dsh), GSK-3β were detected by Western blot. The results showed that TG could reduce the proliferation of HepG2 cells in a concentration dependent manner, with only 31.04% cell viability when TG was 42 μg/mL. In addition, TG could damage the cell structure and induce cell apoptosis, and the apoptosis rate could be as high as 32.44% by AV/PI detection. Moreover, TG could also promote cell necrosis, and limit cell migration. There were significant differences between the treated group and the control group (P<0.05 or P<0.01). Meanwhile, high concentration of TG could arrest HepG2 cells in S and G2/M phase. Finally, compared with the control group, the relative expression of βcatenin and Dsh decreased, while GSK-3β increased in the TG treated groups. In conclusion, TG could inhibit HepG2 cell proliferation by affecting cell cycle progression, promoting apoptosis, and limiting cell migration. The mechanism might be through Wnt/β-catenin signaling pathway, which activated GSK-3β to degrade β-catenin to achieve liver cancer inhibition. © 2023 Science and Technology of Food Industry. All rights reserved.
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页码：389 / 397
页数：8
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