Cloning and Expression Analysis of SOC1 Homologue Gene in Argyranthemum Frutescens

被引：0
|
作者：
Ma Y.-P. [1 ]
Hu J. [1 ]
机构：
[1] College of Life and Health Science, Northeastern University, Shenyang
来源：
Dongbei Daxue Xuebao/Journal of Northeastern University | 2021年 / 42卷 / 04期
关键词：
Argyranthemum frutescens; Cloning; molecular phylogeny; QRT-PCR; SOC1 homologue gene;
D O I：
10.12068/j.issn.1005-3026.2021.04.021
中图分类号：
学科分类号：
摘要：
SOC1 is one of crucial genes in integrating several pathway signals to initiate the transition of flowering. The SOC1 homologue gene AfSOC1 was identified and cloned from Argyranthemum frutescens using RT-PCR and its expression patterns were characterized using qRT-PCR. The results show that AfSOC1 gene consists of a 648-bp open reading frame and encodes a putative protein of 216 amino acids, which is 63.1% identical to SOC1 in Arabidopsis and 62.1% to DEFH68 in Antirrhinum. The putative protein sequences have a typical MADS-box, K-box domain and typical SOC1 motif DVETELFIGP. AfSOC1 was expressed at highly levels in leaves and flower bud. Its expression signals were also detected in shoot, stems, and flowers. Phylogenetic analysis showed that AfSOC1 was most closely related to dicotyledon SOC1-like than to monocotyledon. These results laid a foundation for us to understand the mechanism of AfSOC1 in regulation flowering. © 2021, Editorial Department of Journal of Northeastern University. All right reserved.
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页码：604 / 608
页数：4
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共 30 条
  • [1] Simpson G G, Dean C., Arabidopsis, the Rosetta stone of flowering time? [J], Science, 296, 5566, pp. 285-289, (2002)
  • [2] Boss P K, Bastow R M, Mylne J S, Et al., Multiple pathways in the decision to flower: enabling, promoting, and resetting [J], The Plant Cell, 16, pp. 18-31, (2004)
  • [3] Sung S, Amasino R M., Vernalization and epigenetics: how plants remember winter [J], Current Opinion in Plant Biology, 7, 1, pp. 4-10, (2004)
  • [4] Baurle I, Dean C., The timing of developmental transitions in plants [J], Cell, 125, 4, pp. 655-664, (2006)
  • [5] Parcy F., Flowering: a time for integration [J], International Journal of Development Biology, 49, 5, pp. 585-593, (2005)
  • [6] Lee S, Kim J, Han JJ, Et al., Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO1/AGAMOUS-LIKE 20 (SOC1/ AGL20) ortholog in rice [J], The Plant Journal, 38, 5, pp. 754-764, (2004)
  • [7] Liu C, Zhou J, Bracha-Drori K, Et al., Specification of Arabidopsis floral meristem identity by repression of flowering time genes [J], Development, 134, 10, pp. 1901-1910, (2007)
  • [8] Lee H, Suh S S, Park E, Et al., The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis [J], Gene Development, 14, 18, pp. 2366-2376, (2000)
  • [9] Liu C, Xi W, Shen L, Et al., Regulation of floral patterning by flowering time genes [J], Developmental Cell, 16, 5, pp. 711-722, (2009)
  • [10] Borner R, Kampmann G, Chandler J, Et al., A MADS domain gene involved in the transition to flowering in Arabidopsis [J], The Plant Journal, 24, pp. 591-599, (2000)
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