Cell membrane-coated prussian blue nanoparticles for the detection of tumor cells

A 4T1 cell aptamer programming strategy was constructed to accurately control the capture and release of 4T1 cells on the microplate. In addition, a nano-catalyst PB/PM/HRP/Apt, with good peroxidase (POD) activity, was developed. It can catalyze color developing agent 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to generate blue oxide oxTMB quickly in the presence of H2O2. Under the optimal conditions, the quantitative analysis of 4T1 cells was achieved using colorimetric method, and the absorbance showed a good linear relationship with the concentration of 4T1 cells in the range of 1×104-1×106 cells/mL. This developed therapeutic strategy could be operated simply, and could significantly improve the ability of capturing and quantitatively analyzing for 4T1 cells. © 2024, Chin. J. Anal. Lab. All rights reserved.