Screening and Uric Acid-lowering Potential of Nucleoside-degrading Lactic Acid Bacteria

被引：1
|
作者：
Wang J. [1 ]
Pan L. [1 ]
机构：
[1] Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou
来源：
Shipin Kexue/Food Science | 2022年 / 43卷 / 10期
关键词：
Antiuric acid; Hyperuricemia; Lactobacillus brevis; Probiotics; Xanthine oxidase;
D O I：
10.7506/spkx1002-6630-20210425-356
中图分类号：
学科分类号：
摘要：
In order to obtain special lactic acid bacteria with potent uric acid-lowering activity, this study screened lactic acid bacterial strains for their ability to degrade inosine and guanosine by high performance liquid chromatography (HPLC). The strain LB1lac20 was found to give the highest degradation rate of inosine (91.19%) and guanosine (89.67%).By sequence alignment of 16S rRNA gene using the Basic Local Alignment Search Tool (BLAST), LB1lac20 was identified as Lactobacillus brevis. To evaluate its uric acid-lowering potential, an in vitro model was established by the uric acid production method and used to analyze the inhibitory effect of LB1lac20 on xanthine oxidase. The results showed that the activity of xanthine oxidase was 21.27% and 19.60% inhibited by the live suspension and intracellular extract of LB1lac20, respectively (P <0.05). To sum up, L.brevis LB1lac20 may set the stage for the development of drugs for the prevention and treatment of hyperuricemia. © 2022, China Food Publishing Company. All right reserved.
引用
收藏
页码：199 / 206
页数：7
相关论文
共 21 条
  • [1] DAVIDSON R M, EPPERSON L E., Microbiome sequencing methods for studying human diseases[J], Methods in Molecular Biology, 706, pp. 77-90, (2018)
  • [2] AOUDIA N, RIEU A, BRIANDET R, Et al., Biofilms of Lactobacillus plantarum and Lactobacillus fermentum:effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties[J], Food Microbiology, 53, pp. 51-59, (2016)
  • [3] KIM K T, YANG S J, PAIK H D., Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities[J], Food Science and Biotechnology, 30, 2, pp. 257-265, (2021)
  • [4] CEROVIC M, FORLONI G, BALDUCCI C., Neuroinflammation and the gut microbiota:possible alternative therapeutic targets to counteract Alzheimer's disease?[J], Frontiers in Aging Neuroscience, 11, (2019)
  • [5] MERRIMAN T R, DALBETH N., The genetic basis of hyperuricaemia and gout[J], Joint Bone Spine, 78, 1, (2011)
  • [6] RODDY E, CHOI H K., Epidemiology of gout[J], Rheumatic Disease Clinics of North America, 40, 2, pp. 155-175, (2014)
  • [7] ABELES A M., Hyperuricemia, gout, and cardiovascular disease:an update[J], Current Rheumatology Reports, 17, 3, pp. 1-5, (2015)
  • [8] FEIG D I., Serum uric acid and the risk of hypertension and chronic kidney disease[J], Current Opinion in Rheumatology, 26, 2, (2014)
  • [9] GUO Y, LI H, LIU Z, Et al., Impaired intestinal barrier function in a mouse model of hyperuricemia[J], Molecular Medicine Reports, 20, 4, pp. 3292-3300, (2019)
  • [10] MATSUO H, ISHIKAWA E, MACHIDA H, Et al., Efficacy of xanthine oxidase inhibitor for chronic kidney disease patients with hyperuricemia[J], Clinical and Experimental Nephrology, 24, 7, pp. 307-313, (2019)
123