Screening of α-Glucosidase Inhibitory Peptides from Tea Leaves using Ultrafiltration Affinity Combined with Liquid Chromatography-Mass Spectrometry and Molecular Docking Technology

被引：0
|
作者：
Zan L. [1 ,2 ,3 ,4 ,5 ,6 ]
Wang W. [1 ]
Zhang W. [1 ]
Li X. [1 ,5 ,6 ]
Chen X. [1 ,5 ,6 ]
Yan F. [1 ,5 ,6 ]
Fu J. [1 ,5 ,6 ]
机构：
[1] Shaanxi University of Technology, Hanzhong
[2] Shaanxi Province Key Laboratory of Bio-resources, Hanzhong
[3] Qinba Mountain Area Collaborative Innovation Center of Bioresources Comprehensive Development, Hanzhong
[4] Qinba State Key Laboratory of Biological Resources and Ecological Environment (Incubation), Hanzhong
[5] Tea Planting and Processing Institute, Shaanxi University of Technology, Hanzhong
[6] Shaanxi Provincial Joint Research Center for Tea Industry, Hanzhong
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 18期
关键词：
liquid chromatography-mass spectrometry; molecular docking; tea leaves; ultrafiltration affinity; α-glucosidase inhibitory peptide;
D O I：
10.13386/j.issn1002-0306.2023030066
中图分类号：
学科分类号：
摘要：
Objective: To screen for tea peptides with inhibitory activity against α-glucosidase. Methods: The response surface method was used to optimize the preparation process of tea peptides. Affinity ultrafiltration was used to isolate tea peptides that bind with α-glucosidase, and liquid chromatography-mass spectrometry was used to determine the sequence of the isolated peptides. Virtual screening was performed using bioinformatics methods. Results: The optimal preparation process of tea leaf enzymatic hydrolysis products was alkaline protease hydrolysis temperature of 50 ℃, enzymatic hydrolysis time of 3 h, and a liquid-to-solid ratio of 10:1 (mL/g). The α-glucosidase inhibitory rate was 57.29%. From this, 624 peptide segments were identified, and LIGF was selected for its α-glucosidase inhibitory activity. At a concentration of 5 mg/mL, LIGF exhibited a maximum inhibition rate of 88.13% against α-glucosidase and an IC50 value of 1.22 mg/mL. Molecular docking showed that LIGF could form 5 hydrogen bonds with α-glucosidase, and the binding energy was −3.51 kJ, indicating a high affinity, stability and ability to bind to α-glucosidase. Conclusion: LIGF had potential value as a therapeutic drug for type II diabetes. © 2023 The authors.
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页码：300 / 306
页数：6
相关论文
共 29 条
  • [1] LEHMANN A, HORNBY P J., Intestinal SGLT1 in metabolic health and disease[J], American Journal of Physiology-Gastrointestinal and Liver Physiology, 310, 11, pp. 887-898, (2016)
  • [2] THAO T T P, BUI T Q, HAI N T T, Et al., Newly synthesised oxime and lactone derivatives from Dipterocarpus alatus dipterocarpol as anti-diabetic inhibitors: Experimental bioassay-based evidence and theoretical computation-based prediction[J], RSC Advances, 11, 57, pp. 35765-35782, (2021)
  • [3] CUI Y R., Effect of Dachaihu decoction on oxidative stress induced beta-cell injury of isletin rats with type 2 diabetes, (2019)
  • [4] TIAN W G, LIU Y, GAI X H, Et al., Research progress on mechanism of rehmanniae radix in treatment of type 2 diabetes mellitus[J], Chinese Traditional and Herbal Drugs, 53, 23, pp. 7575-7584, (2022)
  • [5] ZHANG Y, WU F, HE Z, Et al., Optimization and molecular mechanism of novel α-glucosidase inhibitory peptides derived from camellia seed cake through enzymatic hydrolysis[J], Foods, 12, 2, (2023)
  • [6] ZHU Z P, OU Y Q, LIU J Q, Et al., Preparation of antioxidant peptides from proteins in tea residues through ultrasonic-assisted enzymolysis[J], Molecular Plant Breeding, 18, 2, pp. 573-578, (2020)
  • [7] PATIL S P, GOSWAMI A, KALIA K, Et al., Plant-derived bioactive peptides: A treatment to cure diabetes[J], International Journal of Peptide Research and Therapeutics, 26, 2, pp. 955-968, (2020)
  • [8] WANG R, ZHAO H, PAN X, Et al., Preparation of bioactive peptides with antidiabetic, antihypertensive, and antioxidant activities and identification of α-glucosidase inhibitory peptides from soy protein[J], Food Science & Nutrition, 7, 5, pp. 1848-1856, (2019)
  • [9] LIN Y C, HSU P K., Evidence based study of hypoglycemic potential of bitter melon peptide[J], American Journal of Biomedical Science & Research, 9, 1, pp. 60-63, (2020)
  • [10] AYIM I, MA H, ALI Z S, Et al., Preparation of antioxidant peptides from tea (Camellia sinensis L.) residue[J], Journal of food Measurement and Characterization, 12, 3, (2018)
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