Application of a Novel Fluorescent Biosensor Based on Unlabeled Gold Nanoclusters in Rapid Detection of Ochratoxin A

被引：2
|
作者：
Kong D. [1 ]
Luo S. [1 ]
Peng R. [1 ]
Liu J. [1 ]
Wen Q. [1 ]
机构：
[1] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha
来源：
Shipin Kexue/Food Science | 2021年 / 42卷 / 04期
关键词：
Aptamer; Fluorescence resonance energy transfer; Gold nanoclusters; Gold nanoparticles; Ochratoxin A; Toxin;
D O I：
10.7506/spkx1002-6630-20200212-108
中图分类号：
学科分类号：
摘要：
In this study, gold nanoclusters templated with unlabeled nucleic acid aptamers were rapidly synthesized for use in combination with gold nanoparticles modified with single-stranded DNA that is partially complementary to the DNA strands of the aptamer in a novel fluorescent composite nano-biosensor for rapid detection of ochratoxin A. The stronger binding force between the target and aptamer brought about fluorescence resonance energy transfer in the Apt-AuNCs@cDNAAuNPs sensor whose fluorescence had been quenched and consequently recovery of the lost fluorescence intensity, thereby allowing the rapid and accurate detection of ochratoxin A (OTA). The results showed that the peak value of fluorescence intensity (FI) displayed a good linear relationship with the concentration (C) of ochratoxin A within the range from 0.01 to 2.5 ng/mL, which was fitted as FI=689.84lgC + 8 315.31, with the limit of detection (LOD) of 0.025 ng/mL at signal-tonoise ratio of 3. This new fluorescent composite biosensor has the characteristics of simple synthesis, easy operation, high sensitivity, strong selectivity, good stability and a low LOD, which is expected to provide a new idea and platform for the detection of food safety hazard factors. © 2021, China Food Publishing Company. All right reserved.
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页码：263 / 270
页数：7
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