In order to address the current deficit in research on discrete elements within the context of the cotton seed pressing process, a combination of physical tests and simulation experiments were conducted on Xinluzao 84 cottonseed. The Plackett-Burman experimental design was employed to identify the parameters that had a significant impact on the accumulation angle. Subsequently, the Box-Behnken design was employed to establish a second-order regression model for the accumulation angle and the significant parameters. This resulted in the optimal parameter combination for the maximum friction coefficient, minimum sliding friction coefficient and critical shear stress being 0.207, 0.388, and 5x106 Pa, respectively. In order to calibrate the cottonseed bonding parameters, a Box-Behnken test was designed based on the results of the cottonseed particle crushing test. The optimal parameter combinations of normal stiffness, tangential stiffness, and bond radius were obtained as 1.25x109 N/m2, 7.57x108 N/m2, and 0.727 mm, respectively. The obtained parameters were verified by the simulation of stacking angle and the crushing test simulation. The relative error was 0.84% and 0.46%, respectively. This serves to validate the cottonseed bonding model and simulation parameters, thereby providing a foundation for optimising the core structural parameters of the cottonseed shelling machine.