Macroporous adsorption resin for the purification of polyphenols from Aronia melanocarpa berries

In this study, six different macroporous adsorption resins were evaluated for their use to purify polyphenols from Aronia melanocarpa berries. Macroporous resin XAD-7 was selected as the most suitable one. The purification parameters optimized by static adsorption and desorption tests were as follows: adsorption and desorption time, 4 and 2 h, respectively; sample concentration, 3.6 mg/mL; sample pH, 4; and desorption solvent, 95% ethanol (pH 7). The best purification parameters optimized by dynamic adsorption and desorption tests were as follows: sample loading amount, 560 mL; flow rate, 2 mL/min; washing the column with 350 mL of water; and subsequent elution with an eluent volume of 300 mL at 2 mL/min flow rate. Under these conditions, the purity of polyphenols from A. melanocarpa was improved from 11.62% to 64.37%, indicating that macroporous resin XAD-7 has a better effect in purifying polyphenols from A. melancarpa. © 2016, China Food Publishing Company. All right reserved.