Preparation and Structural Characterization of β-Dextranase Hydrolytic Products of Konjac Glucomannan

被引：0
|
作者：
Que F. [1 ,2 ]
Xia Y. [1 ,2 ]
Gao T. [1 ,2 ]
Wang C. [1 ]
Wang L. [1 ,2 ]
Xiong G. [2 ]
Shi L. [2 ]
Ding A. [2 ]
Wu W. [2 ]
机构：
[1] School of Biological Engineering and Food, Hubei University of Technology, Wuhan
[2] Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Wuhan
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 08期
关键词：
chemical properties; enzymatic modification; konjac glucomannan; microstructure;
D O I：
10.7506/spkx1002-6630-20220801-004
中图分类号：
学科分类号：
摘要：
Konjac glucomannan (KGM) was degraded by β-glucanase. The structural characteristics of KGM and its hydrolysate were identified by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), and their molecular masses were determined by gel permeation chromatography with multiple angle laser light scattering. The chemical characteristics of the hydrolysate were evaluated by measuring their intrinsic viscosity, deacetylation degree, soluble polysaccharide content and electrical characteristics. The results showed that the major chain structure of the hydrolysate did not change compared with KGM. After 120 min of treatment with a fivefold excess of the enzyme, a weak crystalline region may appear. SEM results showed that the surface of the hydrolysate gradually became lamellated, and its molecular weight decreased to (36.48 ± 1.23) kDa. The characteristic viscosity decreased by 98%, the degree of deacetylation increased significantly (P < 0.05), and the content of total soluble sugar decreased significantly (P < 0.05). This study can provide a reference for the enzymatic modification of KGM to promote the high-value utilization of KGM hydrolysate resources. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：9 / 15
页数：6
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共 34 条
  • [1] AN K, KANG H, ZHANG L, Et al., Preparation and properties of thermosensitive molecularly imprinted polymer based on konjac glucomannan and its controlled recognition and delivery of 5-fluorouracil, Journal of Drug Delivery Science and Technology, 60, (2020)
  • [2] SHI H B, KHAN I A, ZHONG H T, Et al., Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: edible quality and physicochemical properties of heat-set gel, Food Chemistry, 387, (2022)
  • [3] GAO Y F, LUO C, ZHANG J Y, Et al., Konjac glucomannan improves the gel properties of low salt myofibrillar protein through modifying protein conformation, Food Chemistry, 393, (2022)
  • [4] RAN X L, YANG Z X, CHEN Y F, Et al., Konjac glucomannan decreases metabolite release of a plant-based fishball analogue during in vitro digestion by affecting amino acid and carbohydrate metabolic pathways, Food Hydrocolloids, 129, (2022)
  • [5] ZHANG Y, ZHAO Y, YANG W F, Et al., Structural complexity of konjac glucomannan and its derivatives governs the diversity and outputs of gut microbiota, Carbohydrate Polymers, 292, (2022)
  • [6] JIAN W, TU L, WU L, Et al., Physicochemical properties and cellular protection against oxidation of degraded konjac glucomannan prepared by gamma-irradiation, Food Chemistry, 231, pp. 42-50, (2017)
  • [7] LIU J H, XU Q H, ZHANG J J, Et al., Preparation, composition analysis and antioxidant activities of konjac oligo-glucomannan, Carbohydrate Polymers, 130, pp. 398-404, (2015)
  • [8] ALONSO-SANDE M, TEIJEIRO-OSORIO D, REMUNANLOPEZ C, Et al., Glucomannan, a promising polysaccharide for biopharmaceutical purposes, European Journal of Pharmaceutics and Biopharmaceutics, 72, 2, pp. 453-462, (2009)
  • [9] YANG D, YUAN Y, WANG L, Et al., A review on konjac glucomannan gels: microstructure and application, International Journal of Molecular Sciences, 18, 11, (2017)
  • [10] YE S, ZONGO W S, SHAH B R, Et al., Konjac glucomannan (KGM), deacetylated KGM (Da-KGM), and degraded KGM derivatives: a special focus on colloidal nutrition, Journal of Agricultural and Food Chemistry, 69, 44, pp. 12921-12932, (2021)
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