Dynamic regulation elements and their applications in microbial metabolic engineering

被引：0

作者：

Wu Y. ^{[1
]}

Liu Y. ^{[1
]}

Li J. ^{[1
]}

Du G. ^{[1
]}

Liu L. ^{[1
]}

Chen J. ^{[1
]}

机构：

[1] Key Laboratory of Carbohydrate Chemistry and Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu

来源：

Chen, Jian (jchen@jiangnan.edu.cn) | 2018年 / Materials China卷 / 69期

关键词：

Biochemical engineering; Biotechnology; Dynamic regulation; Metabolism engineering; Regulation elements; Synthetic biology;

D O I：

10.11949/j.issn.0438-1157.20170847

中图分类号：

学科分类号：

摘要：

Metabolic engineering is a key technology for the biosynthesis of various bio-based products such as nutraceuticals, pharmaceuticals, biofuels, and chemicals through the design, construction and optimization of metabolic pathways. Traditional methods, including gene knockout, gene knockdown and gene overexpression usually lead to metabolic imbalance. In contrast, using the regulatory elements derived from the microorganisms themselves to carry out dynamic regulation of metabolic pathways can balance the cell growth and product synthesis, achieving unification of high titer, high yield and high productivity. Microorganisms have the ability to alter their metabolic flux in transcription level by sensing the exchange of environment or intracellular metabolite concentration, and can change the metabolic state using some cis-or trans-regulation elements in post-transcription level, besides allosteric regulation and controlled degradation of pathway enzymes are found in protein level. Dynamic regulation elements can be developed basing on these regulatory mechanisms and can be applied into the dynamic regulation of microbial metabolic. We summarize the dynamic regulation elements in transcription, post-transcription and protein levels respectively, and introduce their applications in microbial metabolic engineering. © All Right Reserved.

引用

页码：272 / 281

页数：9

共 79 条

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