Research on Purification and Antioxidative Activity in Vitro of Peptides Prepared from the British Red Kidney Bean

Kidney bean has very high value of development and utilization, but, a few research were published concerning with preparation and antioxidant activity of kidney bean antioxidant peptides. the protein from the British red kidney bean was taken as raw materials, the kidney bean protein was hydrolyzed by the alkaline protease, the enzymatic hydrolysate were purified through ultrafiltration and Sephadex G-25 gel chromatography. The results showed that, six kinds of antioxidant peptides were obtained, their average molecular weight were 11 407.75, 5 214.35, 4 821.70, 3 311.31, 375.66 and 119.78 u, respectively. The DPPH radical(1, 1-Diphenyl-2-picrylhydrazyl radical) scavenging activity of six antioxidant peptide increased in contrast to their average molecular weight, the 119.78 u DPPH radical scavenging activity of was the highest(84.99%). The total antioxidant capacity, superoxideantion radicals, hydroxyl radicals scavenging activity of 3 311.31 u reached the maximum, it were 215.31 U/mg pro, 251.64 U/mg pro, 181.59 U/mg pro, respectively, and it were closed to the antioxidative activity of ascorbic acid. When the British red kidney bean protein was hydrolyzed by the alkaline protease, the average molecular weights which were from 3 000 u to 5 000 u, had much higher antioxidative activity. © 2018, Editorial Office of Journal of CIFST. All right reserved.