Characterization of Listeria monocytogenes Isolates from Foods by Molecular Subtyping

被引：0

作者：

Wang W. ^{[1
]}

Chen W. ^{[2
]}

Suo Y. ^{[1
]}

Zhou X. ^{[1
]}

Shi C. ^{[1
]}

Shi X. ^{[1
]}

机构：

[1] School of Agriculture & Biology Shanghai Jiao Tong University, MOST-USDA Joint Research Center for Food Safety, State Key Lab of Microbial Metabolism, Shanghai

[2] Technology Center of Bright Dairy & Food Co. Ltd., State Key Laboratory of Dairy Biotechnology, Shanghai

来源：

J. Chin. Inst. Food Sci. Technol. | / 2卷 / 220-226期

关键词：

ERIC-PCR; Food isolates; Listeria monocytogenes; Serotype;

D O I：

10.16429/j.1009-7848.2017.02.029

中图分类号：

学科分类号：

摘要：

Listeria monocytogenes is a major foodborne pathogen, which generally causes human illness including meningitis, meningoencephalitis and abortion. In this study, our aim was to characterize a total of 86 L. monocytogenes food isolates in Shanghai by serotyping and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) subtyping analyses. All the isolates were serotyped by multiplex PCR assays into three serogroups: 1/2a or 3a (72.1%, n=62), 1/2b or 3b or 7 (19.8%, n=17) and 1/2c or 3c (8.1%, n=1). At the relative similarity coefficient of 0.80, 86 isolates and 4 reference strains were identified into 10 clusters and 8 singletons by ERIC-PCR. It was found that the L. monocytogenes isolates sharing the same serogroup could be clustered together by ERIC-PCR at a certain relative similarity coefficient, while isolates belonging to the same serogroup could be efficiently distinguished according to diverse ERIC-PCR profiles. Furthermore, our results will provide the fundamental data for epidemiological surveillance of L. monocytogenes. © 2017, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：220 / 226

页数：6

共 24 条

[1] Mead P.S., Slutsker L., Dietz V., Et al., Food-related illness and death in the United States, Emerging in fectious Diseases, 5, 5, pp. 607-625, (1999)
[2] Lennon D., Lewis B., Mantell C., Et al., Epidemic perinatal listeriosis, Pediatr Infect Dis, 3, 1, pp. 30-34, (1984)
[3] Jemmi T., Stephan R., Listeria monocytogenes: food-borne pathogen and hygiene indicator, Revue Scientifique Et Technique-Office International Des Epizooties, 25, 2, pp. 571-580, (2006)
[4] Multistate outbreak of Listeriosis linked to whole cantaloupes from Jensen farms, Colorado
[5] Liu D.Y., Ainsworth A.J., Austin F.W., Et al., Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes, Journal of Medical Microbiology, 52, 12, pp. 1065-1070, (2003)
[6] Doumith M., Buchrieser C., Glaser P., Et al., Differentiation of the major Listeria monocytogenes serovars by multiplex PCR, Journal of Clinical Microbiology, 42, 8, pp. 3819-3822, (2004)
[7] Casarez E.A., Pillai S.D., Di Giovanni G.D., Genotype diversity of Escherichia coli isolates in natural waters determined by PFGE and ERIC-PCR, Water Research, 41, 16, pp. 3643-3648, (2007)
[8] Khan A.A., Mccarthy S., Wang R.F., Et al., Characterization of United States outbreak isolates of Vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (ERIC) PCR and development of a rapid PCR method for detection of 03:K6 isolates, Fems Microbiology Letters, 206, 2, pp. 209-214, (2002)
[9] Wojciech L., Staroniewicz Z., Jakubczak A., Et al., Typing of Yersinia enterocolitica isolates by ITS profiling, REP- and ERIC-PCR, Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health, 51, 5, pp. 238-244, (2004)
[10] Ye Y., Wu Q., Yao L., Et al., Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii, International Journal of Food Microbiology, 132, 2-3, pp. 172-175, (2009)

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