Multiplex Tandem PCR Gene Disk Assays for the Detection of Genetically Modified Soybean GTS 40-3-2

被引：0
|
作者：
Wei S. [1 ,2 ]
Zhou G. [4 ]
Liu J. [2 ]
Fu W. [3 ]
Zhang Z. [5 ]
Wu X. [1 ]
机构：
[1] Department of Food Science and Engineering, Jinan University, Guangzhou
[2] Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou
[3] Chinese Academy of Inspection and Quarantine, Beijing
[4] Shantou Entry-Exit Inspection and Quarantine Bureau, Shantou, 515041, Guangdong
[5] Department of Food Science & Nutrition, The Chinese University of Hong Kong, HongKong
来源：
Wu, Xiyang (tkentwu@jnu.edu.cn) | 2018年 / Chinese Institute of Food Science and Technology卷 / 18期
关键词：
Detection; Genetically modified organism (GMO); Multiplex tandem polymerase chain reaction (MT-PCR);
D O I：
10.16429/j.1009-7848.2018.02.031
中图分类号：
学科分类号：
摘要：
This study has developed a multiplex tandem polymerase chain reaction (MT-PCR) gene disk assay for the detection of genetically modified soybean GTS 40-3-2. The MT-PCR based on the detection of NOS terminator, CaMV35S promoter, CP4-EPSPS gene, soybean endogenous gene Lectin and event-specific primers of genetically modified soybean GTS 40-3-2. The MT-PCR composed of two PCR rounds: using a small number of cycles (15 cycles) for high-throughput multiplex amplification at first round to avoid competition between amplicons; and then, second round real-time PCR was used to detect individual genes which can be confirmed by melting curve analysis. The MT-PCR assay was rapid (<2 h), high-throughput (5 genes of each sample), sensitive (0.001 ng/μL), and specific in detecting GM soybean GTS 40-3-2. The MT-PCR assay is high-throughput, rapid, and can be used for specific validation of transformation event in transgenic soybean GTS40-3-2. © 2018, Editorial Office of Journal of CIFST. All right reserved.
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页码：244 / 249
页数：5
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