Artesunate induces reactive oxygen species-mediated apoptosis

被引：0
|
作者：
Zhou C. [1 ]
Pan W. [1 ]
Chen T. [1 ]
机构：
[1] Key Laboratory of Laser Life Science, Institute of Laser Life Science, South China Normal University, Guangzhou
来源：
Zhongguo Jiguang/Chinese Journal of Lasers | 2011年 / 38卷 / 02期
关键词：
Apoptosis; Artesunate; Confocal fluorescence microscopy imaging; Medical optics; Mitochondrial membrane potential; Reactive oxygen species;
D O I：
10.3788/CJL20113802.0204003
中图分类号：
学科分类号：
摘要：
It is known that artesunate (ART) induced apoptosis is due to the reactive oxygen species (ROS) generation which triggers many apoptosis. DCFH-DA and Rhodamine 123 were used to probe the level of ROS and mitochondrial membrane potential. Time-lapse confocal fluorescence microscopy was used to monitor the dynamics of ROS generation and the loss of mitochondrial membrane potential during ART-induced human lung adenocarcinoma cells (ASTC-a-1) apoptosis. The data show that the cell ability can be reduced by ART of 0~50 μg/mL. ART of 40 μg/mL which be used to generate significantly ROS can induce notablely loss of mitochondrial membrane potential. Results show that N-acetylcysteine (NAC), a scavenger of ROS, can significantly inhibits the ART-induced apoptosis and the loss of mitochondrial membrane potential and ART induces ROS-mediated apoptosis and loss of mitochondrial membrane potential.
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