Inhibition of Pathogens Adhesion and Internalization to bMEC by Lactic Acid Bacteria

被引：0
|
作者：
Xu N. [1 ,2 ]
Shi G. [1 ]
Zhang H. [1 ]
Du L. [1 ]
Wang J. [2 ]
机构：
[1] Food Science and Engineering College, Shanxi Agricultural University, Taigu, 030801, Shanxi
[2] State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi
来源：
| 2017年 / Chinese Institute of Food Science and Technology卷 / 17期
关键词：
Adhesion; B MEC; Internalization; Lactic acid bacteria;
D O I：
10.16429/j.1009-7848.2017.11.003
中图分类号：
学科分类号：
摘要：
In this study, we tested the ability of Lactococcus lactis CM2 (isolated from mammary gland of healthy cows) and Lactobacillus casei CI2 (isolated from intestinal tract of cows) to prevent invasion of b MEC by two bovine mastitis pathogenic strains, S. aureus SMS81 and E. coli SME177. The results showed that the two lactic acid bacteria strains could inhibit the adhesion and internalization of pathogens in a concentration-dependent manner. Moreover, the inhibition of pathogens adhesion was maintained with heat-killed lactic acid bacterias, whereas live cells was required to prevent pathogens internalization. Also, the culture supernatant of lactic acid bacteria could reduce the adhesion ability of pathogenic cells to b MEC, and this may be related to bacteriocins produced by the strains. Additionally, CM2 significantly up-regulate the antimicrobial peptide TAP and BNBD10 m RNA expression, which maybe involved in the inhibition of pathogens invasion and internalization into b MEC by lactic acid bacteria. Our study will opens avenues for the development of a novel natural, safe and efficient control strategies against bovine mastitis. © 2017, Editorial Office of Journal of CIFST. All right reserved.
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页码：20 / 27
页数：7
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