Antioxidant and Antineoplastic Activities of Carboxymethyled Polysaccharide from Fruits of Phyllanthus emblic L.

被引：0
|
作者：
Cao L. [1 ]
Li L. [1 ]
Wang F. [1 ]
Liao Z. [1 ]
Liu J. [1 ]
Wu L. [1 ]
Wu S. [1 ,2 ]
Wu Y. [3 ]
Li Y. [1 ,2 ]
机构：
[1] College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou
[2] Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou
[3] Qingyuan Agricultural Science and Technology Extension Service Center, Qingyuan, 511518, Guangdong
来源：
Li, Yongyu (yongyu.li@fafu.edu.cn) | 2017年 / Chinese Institute of Food Science and Technology卷 / 17期
关键词：
ABTS; Carboxymethylation; DPPH; HepG-2; NO[!sub]2[!/sub][!sup]-[!/sup; Polysaccharide of emblica;
D O I：
10.16429/j.1009-7848.2017.10.009
中图分类号：
学科分类号：
摘要：
A water-soluble polysaccharide extracted from phyllanthus emblica (EPS) was carboxymethylated in the system of sodium hydroxide-isopropanol-sodium monochloroacetate and the activity of both of the polysaccharide about scavenging free radical, NO2-and inhibiting proliferation effect of HepG-2 as well as cell apoptosis were comparative investigated for the sake of evaluating CM-EPS activity. Characteristics of the spectrum (FT-IR) showed the modification was successful, carboxymethylation of the polysaccharide from emblica (CM-EPS) was prepared. CM-EPS exhibited a declined scavenging effect on ABTS radical but exhibited a markedly enhanced scavenging effect on DPPH in high concentrations compared with the native polysaccharide. In addition, it also revealed an outstanding ability on scavenging NO2- and enhanced inhibitory effect against the proliferation of Human hepatoma cell HepG-2 remarkable. Furthermore, agarose gel electrophoresis demonstated HepG-2 cells were apoptosised with the disposal of CM-EPS which indicated that CM-EPS might be used as a novel functional food for human consumption. © 2017, Editorial Office of Journal of CIFST. All right reserved.
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页码：57 / 63
页数：6
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