Structure-guided development of reversibly photoswitchable green fluorescent proteins probe

被引：0
|
作者：
Wang S. [1 ,2 ,3 ]
Chen X. [1 ,2 ,3 ]
Chang L. [1 ,2 ,3 ]
Xue R. [1 ,2 ,3 ]
Sun Y. [1 ,2 ,3 ]
机构：
[1] State Key Laboratory of Membrane Biology, Peking University, Beijing
[2] Biodynamic Optical Imaging Center, Peking University, Beijing
[3] School of Life Sciences, Peking University, Beijing
来源：
Sun, Yujie (sun_yujie@pku.edu.cn) | 2017年 / Chinese Optical Society卷 / 37期
关键词：
Fluorescent probe; Fluorescent protein; Green fluorescent protein; Microscopy; Optical microscopy; Reversibly photoswitchable; Super-resolution imaging;
D O I：
10.3788/AOS201737.0318002
中图分类号：
学科分类号：
摘要：
In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of live-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by different wavelength lights, thus it can be widely applied in many fields such as high density data optical storage, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on reversible saturable linear fluorescence transitions (RESOLFT) principle. The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs. © 2017, Chinese Lasers Press. All right reserved.
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页数：10
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