Micro-Raman spectra for lipids C-H in colorectal tissue

被引：2

作者：

Gao Z. ^{[1
]}

Yu J. ^{[2
]}

Liu F. ^{[3
]}

Hu B. ^{[2
]}

Guiver M. ^{[4
]}

机构：

[1] College of Biological Engineering, Dalian University, Dalian

[2] Pathology Department, Dalian University Hospital, Dalian

[3] College of Mechanics, Dalian University, Dalian

[4] Institute for Chemical Process and Environmental Technology, National Research Council of Canada, Ottawa, Ontario

来源：

Zhongguo Jiguang/Chinese Journal of Lasers | 2010年 / 37卷 / 02期

关键词：

Cancer; Colorectal tissue; Lipid; Raman spectra; Spectroscopy;

D O I：

10.3788/CJL20103702.0605

中图分类号：

学科分类号：

摘要：

The purpose of this study is to investigate a relationship between the structure of lipid and colorectal carcinogenesis at the molecular level. Colorectal cancer specimens and adjacent normal tissues were measured using confocal Raman microscopy system. The bands at 2857 cm-1and 2891 cm-1originate from CH2 symmetric and asymmetric stretching vibrations, respectively. The band at 2938 cm-1arises from symmetric C-H stretching vibrations. The results show that there are prominent differences between colorectal cancer specimens and adjacent normal tissues in Raman spectroscopy: 1) The intensity of the bands of the cancer tissue spectrum at 2857 cm-1and 2891 cm-1are reduced compared with those of the normal spectra. The relative intensity of the band at 2938 cm-1of lipid of colorectal cancer is stronger than that in normal tissues. 2) The average ratio (I2891/I2857) values is reduced compared with those of the normal spectra. The values for normal and cancerous tissues are 2.21 and 1.69 by the function of 'compare means' of SPSS16.0 software. The research results demostrated that the lateral packing of lipids is loosened which may result in the fluidity of lipid to be increased and the interactions of lipid and protein increase when normal cells are transformed into cancerous cells. Our data suggested there is the relationship between the stretch vibrations of lipids and colorectal carcinogenesis.

引用

页码：605 / 608

页数：3

共 19 条

[1] Wang H., Lu G., Shi Z., Et al., Study on the pathological uterine tissues with a fiber Raman spectrometry, Spectroscopy and Spectral Analisis, 28, 10, pp. 2338-2342, (2008)
[2] Rehman S., Movasaghi Z., Tucker A.T., Et al., Raman spectroscopic analysis of breast cancer tissues: identifying differences between normal, invasive ductal carcinoma and ductal carcinoma in situ of the breast tissue, J. Raman Spectrosc., 38, 10, pp. 1345-1351, (2007)
[3] Kondepati V.R., Keese M., Mueller R., Et al., Application of near-infrared spectroscopy for the diagnosis of colorectal cancer in resected human tissue specimens, Vibration Spectrosc., 44, 2, pp. 236-242, (2007)
[4] Yang J., Ma H., Lu B., Et al., Raman spectroscopy study of phase transformation of TiO<sub>2</sub> rutile single crystal induced by infrared femtosecond laser, Acta Optica Sinica, 27, 10, pp. 1909-1912, (2007)
[5] Liang E., Liang H., Study on the properties and phase transition in laser quenching and cladding layers by Raman spectroscopy, Chinese J. Lasers, 33, 1, pp. 120-123, (2006)
[6] Yu G., Lu A., Wang B., Et al., Study on Raman linear model of human breast tissue, Spectroscopy and Spectral Analisis, 28, 5, pp. 1091-1094, (2008)
[7] Teh S.K., Zheng W., Ho K.Y., Et al., Diagnostic potential of near-infrared Raman spectroscopy in the stomach: differentiating dysplasia from normal tissue, British Journal of Cancer, 98, pp. 457-465, (2008)
[8] Zhang G., Moore J.D., Flach R.C., Et al., Vibrational microscopy and imaging of skin: from single cells to intact tissue, Annal and Bioanal Chem., 387, 5, pp. 1591-1599, (2007)
[9] Krafft C., Codrich D., Pelizzo G., Et al., Raman and FTIR imaging of lung tissue: Methodology for control samples, Vibration Spectrosc., 46, pp. 141-149, (2008)
[10] Robichaux-Viehoever A., Kanter E., Shappell H., Et al., Characterization of Raman spectra measured in vivo for the detection of cervical dysplasia, Applied Spectrosc., 61, 9, pp. 986-993, (2007)

← 1 2 →