Determination of Amantadine and Chloramphenicol Residues in Poultry Meat by Indirect Competitive Chemiluminescence Enzyme-Linked Immunosorbent Assay

被引：0

作者：

Xu X. ^{[1
]}

Su X. ^{[2
]}

Tan S. ^{[2
]}

Zhong C. ^{[2
]}

Zeng D. ^{[2
]}

Zhang Y. ^{[3
]}

Xu Z. ^{[1
]}

Wang X. ^{[1
]}

Yang J. ^{[1
]}

机构：

[1] College of Food Science, South China Agricultural University, Guangzhou

[2] Wens Food Group Co. Ltd., Yunfu

[3] Guangdong Product Quality Supervision and Inspection Institute, Foshan

来源：

Yang, Jinyi (yjy361@163.com) | 1600年 / Chinese Chamber of Commerce卷 / 42期

关键词：

Amantadine; Chloramphenicol; Indirect competitive chemiluminescence enzyme-linked immunosorbent assay; Poultry meat; Pretreatment;

D O I：

10.7506/spkx1002-6630-20191210-110

中图分类号：

学科分类号：

摘要：

An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (ic-CLEIA) was developed for the rapid detection of amantadine and chloramphenicol residues in poultry meat based on efficient sample pretreatment. The dilution factors of coating antigen, antibody and horseradish peroxidase (HRP)-labeled secondary antibody, and competition time were optimized. The results showed that the half-maximum inhibition concentration (IC50) and the linear range were 0.33 μg/L and 0.06-1.77 μg/L for amantadine, and 0.039 μg/L and 0.010-0.179 μg/L for CAP, respectively. Samples were sequentially extracted with ethyl acetate and potassium carbonate solution, and then the extract was blown to dryness with nitrogen gas and purified by n-hexane extraction before being analyzed. The recoveries of amantadine and chloramphenicol from spiked samples were in the ranges of 92.55%-105.14% and 92.00%-112.50%, respectively, and the coefficients of variation were both less than 10.48%. Furthermore, the results determined by ic-CLEIA were highly correlated with those of instrumental analysis method (R2 > 0.98), which demonstrates that the established ic-CLEIA method has high accuracy and good reliability. This method represents a rapid immunoassay for the detection of veterinary drug residues in poultry meat. © 2021, China Food Publishing Company. All right reserved.

引用

页码：305 / 312

页数：7

共 17 条

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