Effect of esaC gene on Edwardsiella tarda's virulence and secretion of T3SS proteins

被引：0

作者：

Li J. ^{[1
,2
]}

Mo Z. ^{[2
]}

Mao Y. ^{[3
]}

Wang B. ^{[1
,2
]}

Yang J. ^{[1
,2
]}

Shao L. ^{[3
]}

机构：

[1] Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Acad. of Sci.

[2] Graduate University of Chinese Acad. of Sci.

[3] College of Marine Life Science, Ocean University of China

来源：

Gaojishu Tongxin/Chinese High Technology Letters | 2010年 / 20卷 / 02期

关键词：

E.tarda; EsaC mutant; Translocator proteins; Type III secretion system (T3SS); Virulence;

D O I：

10.3772/j.issn.1002-0470.2010.02.016

中图分类号：

学科分类号：

摘要：

In order to determine the function of the esaC gene encoding an apparatus protein of the type III secretion system (T3SS) of Edwardsiella tarda, the authors construct a ΔesaC mutant of a pathogenic E.tarda strain LSE40 by an in-frame deletion method. In the mutant nucleotides of 46-1452bp of esaC is deleted. The western blotting analysis shows that the ΔesaC mutant does not secrete the translocator proteins (EseB, EseC and EseD) of E.tarda T3SS into the culture medium. Further investigation shows that the ΔesaC mutant does not autoaggregate in the culture medium. The LD50 (50% lethal dose) of the ΔesaC mutant is shown about 11.5 times higher than that of the wild-type E.tarda in the infection experiments of the blue gourami (Trichogaster trichopterus). These results indicate that esaC affects the secretion of T3SS translocator proteins and the virulence of E.tarda.

引用

页码：199 / 203

页数：4

共 24 条

[1] Ling S.H.M., Wang X.H., Xie L., Et al., Use of green fluorescent protein (GFP) to track the invasive pathways of Edwardsiella tarda in the in vivo and in vitro fish models, Microbiology, 146, 1, pp. 7-19, (2000)
[2] Janda J.M., Abott S.L., Kroshe-Bystrom S., Et al., Pathogenic properties of Edwardsiella species, Journal of Clinical Microbiology, 29, 9, pp. 1997-2001, (1991)
[3] Chen J.D., Huang S.L., Hemolysin from Edwardsiella tarda strain ET16 isolated from eel Anguilla japonica identified as a hole-forming toxin, Fish Science, 62, 4, pp. 542-638, (1996)
[4] Waltman W.D., Shotts E.B., Hsu T.C., Biochemical and enzymatic characterization of Edwardsiella tarda from the United States and Taiwan, Fish Pathology, 21, 1, pp. 1-8, (1986)
[5] Srinivasa Rao P.S., Yamada Y., Leung K.Y., A major catalase (KatB) that is required for H<sub>2</sub>O<sub>2</sub> and phagocyte-mediated killing in Edwardsiella tarda, Microbiology, 149, 9, pp. 2635-2644, (2004)
[6] Tan Y.P., Zheng J., Tung S.L., Et al., Role of type III secretion in Edwardsiella tarda virulence, Microbiology, 151, 7, pp. 2301-2313, (2005)
[7] Grefory V.P., James B.D., Ferraci F., Type III export: New uses for an old pathway, Molecular Microbiology, 40, 2, pp. 284-293, (2001)
[8] Zheng J., Tung S.L., Leung K.Y., Regulation of a type III and a putative secretion system in Edwardsiella tarda by EsrC is under the control of a two-component system, EsrA-EsrB, Infection and Immunity, 73, 7, pp. 4127-4137, (2005)
[9] Zheng J., Li N., Tan Y.P., Et al., EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD, Microbiology, 153, 6, pp. 1953-1962, (2007)
[10] Hueck C.J., Type III protein secretion systems in bacterial pathogens of animals and plants, Microbiology and Molecular Biology Reviews, 62, 2, pp. 379-433, (1998)

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