Cloning and sequencing of beta toxin gene of Clostridium perfringens type C

被引：0
|
作者：
Goswami, P.P. [1 ]
Girish, K.S. [1 ]
Chaudhuri, P. [1 ]
Tiwari, V. [1 ]
Akare, S.J. [1 ]
Harbola, P.C. [1 ]
机构：
[1] National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India
来源：
Indian Journal of Experimental Biology | 2002年 / 40卷 / 01期
关键词：
Reaction kinetics;
D O I：
暂无
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学科分类号：
摘要：
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfringens.
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页码：109 / 110
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