Influence of transplantation of bone mesenchymal stem cells on the acute injury motor function of the spinal cord and expression of the nerve growth factor of the rat

Purpose to research the therapeutic affect of the allograft of Bones Mesenchymal Stem Cells (BMSCs) on the acute injury of the spinal nerve of the rat. Method: Take 1 Westar healthy rat, collect the bone marrow, adopt the adherence method to separate BMSCs and culture and mark them, cultivate the BMSCs culture solution with the cell population of about 5H104 uL-1 for transplantation. Establish 40 westar rat models with the acute injury of the spinal cord, which shall be divided as the transplantation group and the control group, 20 pieces for each group. After a week of injury, inject BMSCs slowly to the injury center of the rat's spinal cord, inject the physiological saline to the control group and observe and inspect the rehabilitation efficacy of the hind limb function and the protein expression of the Nerve Growth Factor (NGF) and Brain-derived Neurotrophic Factor (BDNF) of the rats of two groups. Result: The rehabilitation efficacy of the hind limb function of the transplantation group is obvious better than that of the control group after 3-8 weeks of injury and the difference is of significance (p