Modified Icariin covalent with chitosan/poly hydroxybutyrate-co-hydroxyvalerate to fabricate tissue inducible bone repair scaffold material

In order to fabricate an inducible bone repair scaffold materials, Icariin (Ica) were modified by amino and then got Ica-NH2, Ica-NH2 was characterized by FTIR, XRD and TGA. Ica-NH2 covalent binding with carboxymethyl chitosan (CS) as the inducing factor addition method, poly hydroxybutyrate-co-hydroxyvalerate (PHBV) and CS were used as basic materials, then two-phase mixed rapid freezing/freeze-drying technology was used to build the Ica-NH2-CS/PHBV bone tissue engineering scaffolds. And then the scaffold materials were evaluated by SEM, invitro drug release, mechanical properties, cell compatibility and proliferation. The results show that: there were two moderate intensity, sharp N-H stretching vibration peaks at 3371 and 3328 cm-1 in the FTIR spectra of aminated Ica, one N-H waving vibration peaks appears at 1689 cm-1, XRD pattern shows the diffractions are enhanced and move to the left as a whole, these results indicate that Ica is successfully amino-modified. The scaffold materials possess network- and bead-like mircostructures, which are uniformly distrubuted wtih microporous of 3-10 μm. Ica-NH2-CS/PHBV scaffold has good invitro drug release effect. Mechanical strength tests show its mechanical strength is between dense bone and cancellous bone, composite cells closely adhere on the scaffold after 7 d cultivation and proliferate well, and the proliferation rate of cells attached on Ica-NH2-CS/PHBV scaffold materials is significantly higher than that on the CS/PHBV. This research indicates that Ica-NH2-CS/PHBV scaffold materials could be used as good type of inducible bone repair materials. © 2016, BUAA Culture Media Group Ltd. All right reserved.