Study on water-soluble benzylidene cyclopentanone dyes for two-photon induced photodynamic therapy

被引：0
|
作者：
Yang W. [1 ,3 ]
Zou Q. [1 ,3 ]
Zhou Y. [2 ]
Gu Y. [2 ]
Zhao Y. [1 ]
Wu F. [1 ]
机构：
[1] Technical Institute of Physics and Chemistry, Chinese Acad. of Sci.
[2] Department of Laser Medicine, Chinese People's Liberation Army General Hospital
[3] Graduate University of Chinese Acad. of Sci.
来源：
Guangxue Xuebao/Acta Optica Sinica | 2011年 / 31卷 / SUPPL.1期
关键词：
Benzylidene cyclopentanone; Medical optics; Photodynamic therapy; Reactive oxygen species; Two-photon excitation;
D O I：
10.3788/AOS201131.s100118
中图分类号：
学科分类号：
摘要：
Four water-soluble benzylidene cyclopentanone dyes are synthesized for photodynamic therapy (PDT) by introducing different numbers of carboxylate groups on their periphery. Their photophysical and photochemical properties are investigated systematically. The results show that the water solubility of these dyes is significantly enhanced after modification. All dyes exhibit large two-photo absorption (TPA) cross-section in near infrared (IR) range, which can generate both superoxide anion radical and singlet oxygen species under visible illumination. One-photon PDT experiments indicate that four novel dyes displaye low dark cytotoxicity. However, only the dye Y1 presents excellent photodynamic activity, which highlights the role of moderate lipophilicity for PDT dyes. Further cellular experiments reveals that Y1 had obvious two-photon induced cytotoxic effect on human rectal cancer 1116 cells. The result indicates that Y1 is a potential photosensitizer for two-photon induced PDT.
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  • [1] MacDonald I.J., Dougherty T.J., Basic principles of photodynamic therapy, J. Porphyrins Phthalocyanines, 5, 2, pp. 105-129, (2001)
  • [2] Di J., Zhou M., Yang H., Et al., Manufacturing micro-biological device and scaffold research with two-photon femtosecond laser technology, Chinese J. Lasers, 36, 1, pp. 249-254, (2009)
  • [3] Guo F., Hu Z., Fu X., Et al., Photopolymerization behaviors of novel two-photon absorption initiators and fabrication of microdevices, Chinese J. Lasers, 36, 6, pp. 1528-1534, (2009)
  • [4] Li Z., Huang Z., Chen R., Et al., Two-photon fluorescence imaging of thyroid tissue, Chinese J. Lasers, 36, 3, pp. 765-768, (2009)
  • [5] Atif M., Dyer P.E., Paget T.A., Et al., Two-photon excitation studies of m-THPC photosensitizer and photodynamic activity in an epithelial cell line, Photodiagn. Photodyn. Ther., 4, 2, pp. 106-111, (2007)
  • [6] Khurana M., Collins H.A., Karotki A., Et al., Quantitative in vitro demonstration of two-photon photodynamic therapy using Photofrin and Visudyne, Photochem. Photobiol., 83, 6, pp. 1441-1448, (2007)
  • [7] Wu J., Zhao Y.X., Li X., Et al., Multibranched benzylidene cyclopentanone dyes with large two-photon absorption cross-sections, New J. Chem., 30, 6, pp. 1098-1103, (2006)
  • [8] Xue J.Q., Zhao Y.X., Wu J., Et al., Novel benzylidene cyclopentanone dyes for two-photon photopolymerization, J. Photochem. Photobiol. A: Chem., 195, 2, pp. 261-266, (2008)
  • [9] Diwu Z.J., Lown J.W., Photosensitization by anticancer agents 12. Perylene Quinonoid pigments, a novel type of singlet oxygen sensitizer, J. Photochem. Photobiol. A: Chem., 64, 3, pp. 273-287, (1992)
  • [10] Xu C., Webb W.W., Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm, J. Opt. Soc. Am. B, 13, 3, pp. 481-491, (1996)
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