Structural Modification and Solubility Enhancement of Glutenin by pH-Shifting

被引：1
|
作者：
Rong Y. [1 ]
Hou Y. [1 ]
Cao X. [1 ]
Zhang J. [1 ]
Zhou B. [1 ,2 ]
机构：
[1] School of Biological Engineering and Food, Hubei University of Technology, Wuhan
[2] Key Laboratory of Fermentation Engineering, Ministry of Education, Wuhan
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 20期
关键词：
glutenin; pH-shifting treatment; protein structure; solubility; surface hydrophobicity;
D O I：
10.7506/spkx1002-6630-20230209-088
中图分类号：
学科分类号：
摘要：
Glutenin (Glu) was modified by pH-shifting to improve its properties. The effect of pH-shifting treatment on the structure and micromorphology of glutenin was investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM), fluorescence spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The results showed that pH-shifting could significantly reduce the particle size of Glu aggregates and increase the surface charge of Glu granules. Meanwhile, it increased the hydrophobicity and sulfhydryl group content, and decreased the disulfide bond content, and the effect of alkaline pH-shifting was more significant than that of acidic pH-shifting. In addition, alkaline pH-shifting significantly improved the solubility of Glu, which could play an active role in broadening the application range of Glu in the food industry. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：20 / 27
页数：7
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