Cloning and expression of type III polyketide synthase gene in Fallopia multiflora

被引：0
|
作者：
Sheng S.-J. [1 ]
Zhao S.-J. [2 ]
机构：
[1] School of Biological Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong
[2] General Hospital of Guangzhou Military Command, Guangzhou 510010, Guangdong
来源：
Huanan Ligong Daxue Xuebao/Journal of South China University of Technology (Natural Science) | 2010年 / 38卷 / 05期
关键词：
Bioinformatics; Cloning; Expression; Fallopia multiflora; Polyketide synthase;
D O I：
10.3969/j.issn.1000-565X.2010.05.028
中图分类号：
学科分类号：
摘要：
In order to regulate the secondary metabolic pathway of a Chinese traditional medicinal material Fallopia multiflora by genetic engineering technology, a gene named FmPKS2 (GenBank accession number: GQ984139) was cloned via the rapid amplification of complementary DNA end and bioinformatics analysis. The gene consists of 1498bp nucleotides for coding sequence, encodes 392 amino acids with a predicted relative molecular mass of 43160 and an isoelectric point of 5.85, and contains several highly hydrophobic regions with a hydrophobic coefficient of -0.154. Phylogenetic analysis reveals that FmPKS2 shares the closed relationship with chalcone synthase of polygonaceae family. The transcription of FmPKS2 can be found in all different tissues of Fallopia multiflora, with the highest quantity in rhizomes.
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页码：144 / 148
页数：4
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