Large Eddy Simulation (LES) of fully developed turbulent channel flow with heat transfer was performed to investigate the effects of the Reynolds number on the turbulence behavior. In the present study, the bottom wall of the channel was cooled and the top wall was heated. The Reynolds numbers, based on the central mean-velocity and the half-width of the channel, were chosen as 4000, 6000, 104 and 2×104, and the Prandtl number as 1.0. To validate the calculations, the present results were compared with available data obtained by Direct Numerical Simulation (DNS), which proves to be in good agreement with each other. To reveal the effects of the Reynolds number, some typical quantities, including the velocity fluctuations, temperature fluctuation, heat fluxes and turbulent Prandtl number, were studied.