Microwave-assisted method for extracting chlorophyll-a in phytoplankton and its comparison with freezing-thawing extraction method

被引：0
|
作者：
Lin L. [1 ]
Tang Q. [1 ]
Peng L. [1 ]
Wei G. [1 ]
Lin S. [2 ]
Li X. [2 ]
Yang H. [2 ]
机构：
[1] Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Department of Ecology, Jinan University, Guangzhou
[2] Hydrological Bureau of Guangdong Province, Guangzhou
来源：
Wei, Guifeng (tweigf@jnu.edu.cn) | 1600年 / Science Press卷 / 28期
关键词：
Chlorophyll-a; Extraction; Freezing-thawing method; Microwave-assisted extraction method; Phytoplankton;
D O I：
10.18307/2016.0526
中图分类号：
学科分类号：
摘要：
In order to save time of sample processing and to improve the determining accuracy, a microwave-assisted extraction method was designed and optimized to extract chlorophyll-a in phytoplankton, and was compared to the freezing-thawing method for extraction efficiency of chlorophyll-a. The results showed that microwave-assisted extraction method was optimal in 60 s treatment with output power of 800 W, and the appropriate water volume for filtration was about 100-500 ml for samples collected in meso-eutrophic waterbodies and 1000 ml for those from oligotrophic waterbodies. The chlorophyll extraction rate of microwave-assisted extraction method was significantly higher than that of freezing-thawing method in meso-eutrophic waterbodies, which can increased by 7%-12%, while freezing-thawing method was more stable in measuring chlorophyll-a of oligotrophic waterbodies. For those algae with a glue shell or siliceous shell, microwave-assisted extraction method had a higher efficiency for chlorophyll-a extraction and a lower relative standard deviation than the freeze-thaw method. The microwave-assisted extraction method needs only one half operation time of the freezing-thawing extraction method, so that it is more suitable for emergency monitoring of eutrophic waterbodies, while the freezing-thawing extraction method is more suitable for monitoring oligotrophic waterbodies. © 2016 by Journal of Lake Sciences.
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页码：1148 / 1152
页数：4
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