Studies on the interactions between Pto and Pti1s proteins from maize by a yeast two-hybrid system

To check whether the protein ZmPto can interact with the protein ZmPti1s, in this study, the gene sequences of ZmPto and ZmPti1a, ZmPti1b were inserted into yeast expression vectors pEG202 and pJG4-5, respectively. Yeast two-hybrid was then conducted to verify their interactions. Results showed that yeast strains neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPti1 or ZmPto can activate reporter genes. Only the yeast strains carrying both fusion sequence of ZmPti1 and ZmPto can activate reporter genes indicating that ZmPto can interact directly with ZmPti1 in the yeast two-hybrid system. © Maxwell Scientific Organization, 2012.