Studies on the Antioxidant Activity and Distribution in Gastrointestinal Tract of the Polysaccharide Hydrolysate from Auricularia auricula

被引：0

作者：

Xu Y. ^{[1
]}

Xu S. ^{[1
]}

Wang S. ^{[1
]}

He J. ^{[1
]}

Zhang Y. ^{[1
]}

Wang G. ^{[1
]}

Wang W. ^{[1
]}

机构：

[1] College of Life Sciences, China Jiliang University, Hangzhou

来源：

Zhang, Yongjun (yjzhang@vip.163.com) | 2018年 / Chinese Institute of Food Science and Technology卷 / 18期

关键词：

Antioxidation; Auricularia auricular polysaccharide; Distribution in gastrointestinal tract; Fluorescent labeling; Simulated degradation;

D O I：

10.16429/j.1009-7848.2018.03.003

中图分类号：

学科分类号：

摘要：

The purpose of this study was to investigate the antioxidant activity and the dynamic distribution of the digestive tract in mice of the Auricularia auricular polysaccharide fragments from the artificial gastrointestinal fluid in vitro. The 5 kinds of polysaccharides (AAPF-1, AAPF-2, AAPF-3, AAPF-4, AAPF-5) were separated from Auricularia auricular by alkali extraction and alcohol precipitation, deproteinization, artificial gastrointestinal fluid hydrolysis, dialysis and gel column chromatography (Sephadex G100). OH• and DPPH• clearance in vitro and nematode H2O2 resistance experiment of those 5 kinds of polysaccharides were analyzed. AAPF-1-Tyr-FITC was obtained by fluorescein labeled, and the dynamic distribution in the gastrointestinal tract of mice was studied. Results showed that AAPF-1 (0.4 mg/mL) for OH• and DPPH• removal rate was the highest among 5 kinds of polysaccharides, that was 68.54% and 42.18% respectively, and the life span of the nematodes was significantly prolonged within 60~150 min. AAPF-1 was quickly evacuated to the small intestine, and not absorbed in the small intestine in mice, and the remaining time in the large intestine was more than 24 h. © 2018, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：16 / 22

页数：6

共 5 条

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