Analysis of Fungal Diversity of Moldy Walnut Kernel Based on High-throughput Sequencing Technology

被引：0

作者：

Fang Y. ^{[1
]}

Su G. ^{[2
]}

Wang W. ^{[3
]}

Bai Y. ^{[2
]}

Wang F. ^{[1
]}

Pei D. ^{[2
]}

机构：

[1] State Key Laboratory of Efficient Production of Forest Resources, Beijing Key Laboratory of Forest Processing and Safety, College of Biological Sciences and Technology, Beijing Forestry University, Beijing

[2] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing

[3] College of Life Sciences, Northeast Forestry University, Harbin

来源：

Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 08期

关键词：

fungal community diversity; fungi; high-throughput sequencing; mildew; walnut;

D O I：

10.16429/j.1009-7848.2023.08.036

中图分类号：

学科分类号：

摘要：

Objective: To reveal the changes of fungal community structure and diversity in walnut the potential health threats during natural mildew through studying the fungal infection process of walnut during natural mildew. Methods: The fungal ITS rDNA of fresh walnut kernel samples with different mildew time was sequenced by Illumina Miseq high - throughput sequencing technique, and the changes of fungal community composition and diversity in the process of fresh walnut kernel mildew were analyzed. Results: Ascomycota is the main fungal species in the mildew process of walnut samples, with a relative abundance of 27.41%-99.98%, followed by Basidiomycota with a relative abundance of 0.01%-32.56%. At the fungal level, it was found that the main dominant populations in the mildew process were Fusarium, Alternaria, Gibberella and Aspergillus. The mildew process of walnut in a week can be divided into three periods: Period I (0-2 days), Period II (3-4 days) and Period M (5-7 days). In stages of Period I and Period II, Fusarium was the dominant genus, and the main fungus that change significantly among other periods were Fusarium, Aspergillus, Alternaria, Gibberella and Penicillium. Conclusion: The changes of fungal microbial community during mildew of walnut could be divided into three periods, during which the fungal diversity showed a downward trend, and there were a variety of harmful pathogens, such as Fusarium and Aspergillus, etc., which might cause potential safety risks if not handled properly. © 2023 Journal of Chinese Institute of Food Science and Technology. All rights reserved.

引用

页码：369 / 378

页数：9

共 40 条

[1] QI J., Study on walnut nut quality in main growing areas of China [D], (2009)
[2] GUO W., Preliminary study on quality change and toxin identification of grain mildew process [D], (2011)
[3] LIU J, ZHANG L Z, CHEN L L, Et al., Fungal diversity analysis of Camellia oleifera seeds during storage, Journal of the Chinese Cereals and Oils Society, 33, 9, pp. 85-92, (2018)
[4] PARENTE E, RICCIARDI A, ZOTTA T., The microbiota of dairy milk: A review, International Dairy Journal, 107, 1, (2020)
[5] ZHANG Y J, SKAAR I, SULYOK M, Et al., The microbiome and metabolites in fermented Pu-erh tea as revealed by high -throughput sequencing and quantitative multiplex metabolite analysis, PLoS One, 11, 6, (2016)
[6] CHEN Y W, WU B, ZHANG C, Et al., Current progression: Application of high-throughput sequencing technique in space microbiology, Bio Med Research International, 2020, 3, pp. 1-13, (2020)
[7] SONG W F, LI M C, GAO Z., Research progress on in situ detection methods of microorganisms, Biotechnology Bulletin, 33, 10, pp. 26-32, (2017)
[8] CAO C X, HAN W, ZHANG H P., Application of third generation sequencing technology to microbial research [J], Microbiology China, 43, 10, pp. 2269-2276, (2016)
[9] ZHANG W Q, MA H P, LIU C Y, Et al., High-throughput sequencing analysis of yak yoghurt fungal diversity under different collection and storage temperatures in Gannan grazing area, Science and Technology of Food Industry, 42, 13, pp. 163-169, (2021)
[10] CHEN Q L, LI Z, WANG H C, Et al., Fungal community composition and diversity analysis of tobacco leaf with different mildew degree after curing [J], Acta Microbiologica Sinica, 59, 12, pp. 2401-2409, (2019)

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