Characterization of Agronomy, Grain Physicochemical Quality, and Nutritional Property of High-Lysine 35R Transgenic Rice with Simultaneous Modification of Lysine Biosynthesis and Catabolism

被引：0

作者：

机构：

[1] [1,Yang, Qingqing

[2] Wu, Hongyu

[3] 1,Li, Qianfeng

[4] Duan, Ruxu

[5] 1,Zhang, Changquan

[6] 2,Sun, Samuel Saiming

[7] 1,Liu, Qiaoquan

来源：

Sun, S.S. (ssun@cuhk.edu.hk) | 1600年 / American Chemical Society卷 / 65期

基金：

中国国家自然科学基金;

关键词：

Essential amino acids - Feeding experiments - Field performance - free lysine - Nutritional properties - Physico-chemical quality - Protein efficiency ratio - Transgenic rice;

D O I：

暂无

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学科分类号：

摘要：

Lysine is the first limiting essential amino acid in rice. We previously constructed a series of transgenic rice lines to enhance lysine biosynthesis (35S), down-regulate its catabolism (Ri), or simultaneously achieve both metabolic effects (35R). In this study, nine transgenic lines, three from each group, were selected for both field and animal feeding trials. The results showed that the transgene(s) caused no obvious effects on field performance and main agronomic traits. Mature seeds of transgenic line 35R-17 contained 48-60-fold more free lysine than in wild type and had slightly lower apparent amylose content and softer gel consistency. Moreover, a 35-day feeding experiment showed that the body weight gain, food efficiency, and protein efficiency ratio of rats fed the 35R-17 transgenic rice diet were improved when compared with those fed wild-type rice diet. These data will be useful for further evaluation and potential commercialization of 35R high-lysine transgenic rice. © 2017 American Chemical Society.

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