Genetically encoded cleavable protein photo-cross-linker

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机构：
[1] Lin, Shixian
[2] He, Dan
[3] Long, Teng
[4] Zhang, Shuai
[5] Meng, Rong
[6] 1,Chen, Peng R.
来源：
Chen, P.R. (pengchen@pku.edu.cn) | 1600年 / American Chemical Society卷 / 136期
关键词：
We have developed a genetically encoded; selenium-based cleavable photo-cross-linker that allows for the separation of bait and prey proteins after protein photo-cross-linking. We have further demonstrated the efficient capture of the in situ generated selenenic acid on the cleaved prey proteins. Our strategy involves tagging the selenenic acid with an alkyne-containing dimethoxyaniline molecule and subsequently labeling with an azide-bearing fluorophore or biotin probe. This cleavage-and-capture after protein photo-cross-linking strategy allows for the efficient capture of prey proteins that are readily accessible by two-dimensional gel-based proteomics and mass spectrometry analysis. © 2014 American Chemical Society;
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