Genetic Diversity of Chinese Fir (Cunninghamia lanceolata) Breeding Populations among Different Generations

被引：0
|
作者：
Li X. [1 ,2 ]
Wang L. [3 ]
Wen Y. [1 ]
Lin J. [4 ]
Wu X. [1 ]
Yuan M. [1 ]
Zhang Y. [1 ]
Wang M. [1 ]
Li X. [1 ,2 ]
机构：
[1] College of Landscape Architecture, Central South University of Forestry and Technology, Changsha
[2] Guangxi Dongmen Forest Farm, Chongzuo
[3] College of Forestry, Central South University of Forestry and Technology, Changsha
[4] Longshan State Forest Farm of Lechang, Lechang
来源：
Linye Kexue/Scientia Silvae Sinicae | 2020年 / 56卷 / 11期
关键词：
Breeding populations; Cunninghamia lanceolata; Genetic diversity; Microsatellite markers;
D O I：
10.11707/j.1001-7488.20201106
中图分类号：
学科分类号：
摘要：
Objective: The aim of this study was to analyze the genetic diversity of Chinese fir(Cunninghamia lanceolata) breeding populations, and to detect its genetic structure and gene flow, further to provide a scientific basis for long term breeding and sustainable management of Chinese fir. Method: Using nuclear and chloroplast microsatellite markers, a total of 520 clones of 2 breeding populations respectively of two national seed bases(Youxian and Lechang)were analyzed to compare their genetic diversity among different generations. Result: nSSR data showed that total genetic diversity of breeding populations was 0.687. Youxian and Lechang seed bases displayed a high genetic diversity, with expected heterozygosity of 0.678 and 0.667, respectively. With the advancement of breeding process, genetic diversity of advanced-generations of the two seed bases tended to decrease gradually. The average number of alleles in the two bases (Youxian and Lechang) decreased from 9.612 (F1) to 8.750 (F2), the F3 generation of the Lechang Base had the smallest allele number 6.833, the expected heterozygosity was decreased by less than 6.46% among generations. cpSSR markers also showed a clear change of genetic diversity, but the changing trend was different in the two seed bases among different generations. With the advancement of breeding process, genetic diversity of the Youxian Base gradually increased, while Lechang Base showed a trend of a decline followed by a rise. This difference is related to the evolution model of chloroplast and nuclear genomes, and also affected by the number and sources of breeding materials in the two seed bases. Conclusion: Chinese fir breeding populations in the two national seed bases have high level of genetic diversity. With the advancement of breeding process, genetic diversity of advanced-generation breeding populations was decreasing gradually, but the reduction rate was less than 6.46%, suggesting that the advanced-generation breeding strategies and methods of Chinese fir were scientifically sound and effective. © 2020, Editorial Department of Scientia Silvae Sinicae. All right reserved.
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页码：53 / 61
页数：8
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