Determination of mildronate and related substances by HPLC-ELSD

被引：0
|
作者：
Cao, Guo-Ying [1 ]
Lü, Run-Feng [2 ]
Hu, Xin [1 ]
Sun, Chun-Hua [1 ]
机构：
[1] Department of Pharmacy, Beijing Hospital, Beijing 100730, China
[2] Shandong Medical University, Jinan 250012, China
来源：
Chinese Pharmaceutical Journal | 2005年 / 40卷 / 11期
关键词：
Acetonitrile - Assays - High performance liquid chromatography - Separation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
OBJECTIVE: To establish a method for the determination of mildronate and related substances. METHODS: Using HPLC-ELSD method, the related substances were separated on a CN - 3 column(4.6 mm × 250 mm, 5 μm, Inertsil), and the mobile phase consisted of water and acetonitrile(80:20). The ELSD detection temperature was 90 °C, and the gas rate was 2.5 mL&middotmin-1. RESULTS: The liner range was 10.3-814.4 μg&middotmL-1. The RSD of precision was 0.41% for intra-run validation and 1.63% for inter-run validation. CONCLUSION: This method was simple and accurate to determine mildronate and the related substance.
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页码：864 / 866
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