Optimization of the extraction process of peristrophe roxburghiana polysaccharide and analysis of its antioxidant activity

被引：0
|
作者：
Chen Y. [1 ]
Song Z. [1 ]
Wu H. [2 ]
Su X. [1 ]
机构：
[1] School of Chemistry and Biological Engineering, Guangxi Normal University for Nationalities, Chongzuo
[2] School of Food Science and Technology, South China University of Technology, Guangzhou
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 05期
关键词：
Antioxidant; Extraction process; Peristrophe roxburghinana; Polysaccharide; Response surface optimization method;
D O I：
10.13386/j.issn1002-0306.2022050191
中图分类号：
学科分类号：
摘要：
In order to explore the optimum extraction process of polysaccharide and antioxidant activity in vitro in Peristrophe roxburghiana. In this study, Peristrophe roxburghiana was used as raw material to explore the effects of solid-liquid ratio, extraction temperature, time and frequency on the yield of polysaccharide. The extraction process of polysaccharide in Peristrophe roxburghiana was optimized by response surface methodology and the antioxidant activity of the polysaccharide was investigated by measuring its scavenging ability on DPPH·, ·OH, ABTS+· and other free radicals. The results showed that we could get the most quantity of polysaccharide extracted from Peristrophe roxburghiana, which reaches up to 11.05%, in these conditions: The solid-liquid ratio was 1:31 g/mL, the extraction temperature was 85 ℃, the extraction time was 118 min and the extraction frequency was 3 times. The scavenging ability of the polysaccharide against free radical was quantitatively related to the concentration of polysaccharide within a certain range. The IC50 values of DPPH·, ·OH and ABTS+· radicals scavenged by the polysaccharide solution were 0.18, 0.73 and 0.64 mg/mL, respectively, indicating that the polysaccharide extracted from Peristrophe roxburghiana was with certain antioxidant activity. By exploring the optimal process and antioxidant activity of the polysaccharide extracted from Peristrophe roxburghiana, we provide a theoretical basis and reference for the further development and application of the polysaccharide in the future. © 2023, Editorial Department of Science and Technology of Food Science. All rights reserved.
引用
收藏
页码：166 / 173
页数：7
相关论文
共 30 条
  • [1] YUAN F, LI L, LI X, Et al., Optimization of purple glutinous rice processing technology based on fuzzy mathematics sensory evaluation method[J], Cereals & Oils, 34, 6, pp. 112-117, (2021)
  • [2] QI B W, CHEN L H., Study on the optimization of extraction process and characteristics of red pigment from eccoilopus cotulifer by response surface method[J], China Condiment, 46, 5, pp. 151-160, (2021)
  • [3] WEI Z, LAI H F, PENG L J, Et al., Optimization of extraction process of total phenolic acids from the Peristrophe baphica (spreng.) bremek by response surface methodology and its antioxidation response surface methodology and its antioxidation[J], Food Research and Development, 41, 18, pp. 84-90, (2020)
  • [4] WEI Z, HUANG X X, LAI H F., Review on chemical composition, pharmacological activity and application of Peristrophe baphica (spreng) bremek[J], Natural Product Research and Development, 28, 12, pp. 2035-2043, (2016)
  • [5] CHEN W B, WANG W P, ZHAN H S, Et al., Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes[J], Carbohydrate Polymers, 15, pp. 446-455, (2018)
  • [6] CHEN S F, LI Q T, HUANG K, Et al., Optimization of extraction process of polysaccharide from Merremia hederacea and its antioxidant activity[J], Applied Chemical Industry, 50, 11, pp. 3031-3035, (2021)
  • [7] ZENG K F, CHEN W B, PING H, Et al., Structural characterization of polysaccharides with potential antioxidant and immunomodulatory activities from Chinese water chestnut peels[J], Carbohydrate Polymers, 87, pp. 614-619, (2020)
  • [8] CHEN Y, WU H., Experimental techniques for structural characterization and immune activity of polysaccharides, pp. 18-27, (2022)
  • [9] CHEN W B., Structural elucidation and the biological activity of polysaccharides from Saussurea laniceps, (2020)
  • [10] ZHANG H, ZOU P, ZHAO H, Et al., Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis[J], Carbohydrate Polymers, 251, (2021)
123