Polymer Screen and Preparation of the Thermo-Responsive Fluorescent Nanospheres

被引：0
|
作者：
Sheng Y. [1 ,2 ]
Duan Z. [1 ]
Sun Y. [1 ,2 ]
Li J. [1 ,2 ,3 ]
Zhang R. [1 ,2 ,3 ]
机构：
[1] School of Materials Science and Engineering, Changzhou University, Changzhou
[2] Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou
[3] Jiangsu Collaboration Innovation Center of Photovoltaic Science and Engineering, Changzhou
来源：
Gaofenzi Cailiao Kexue Yu Gongcheng/Polymeric Materials Science and Engineering | 2018年 / 34卷 / 11期
关键词：
Biocompatibility; Cellular imaging; Fluorescent conjugated polymer; Fluorescent intensity; Polymer microarray;
D O I：
10.16865/j.cnki.1000-7555.2018.11.023
中图分类号：
学科分类号：
摘要：
In this work, acrylate polymer microarrays encapsulated with a fluorescent conjugated polymer (FCP) were prepared by inkjet printing approach followed with UV initiated in situ polymerization. The fluorescent intensity of each spot on the arrays was analyzed for the identification of the monomer compositions that enhanced the fluorescent intensity of the embedded FCP. The identified polymer candidates were then up-scaled by synthesizing fluorescent polymer nanospheres. The chemical composition and fluorescent properties were analyzed by FT-IR, UV-vis, SEM, TEM and fluorescence spectroscopy. The as-synthesized nanospheres show reversible fluorescent intensity change following switching of temperature, higher temperature makes fluorescent intensity weaker and the fluorescent intensity is recovered as temperature reducing. In vitro cell viability assay shows that the nanospheres loaded with FCP are cell compatible with low cytotoxicity. Therefore, they are promising to be used as fluorescent probes for cellular imaging and tracking. © 2018, Editorial Board of Polymer Materials Science & Engineering. All right reserved.
引用
收藏
页码：137 / 143and150
相关论文
共 12 条
  • [1] Wang G.R., Duan Z.Q., Sheng Y., Et al., Tuning the emission properties of a fluorescent polymer using a polymer microarray approach-identification of an optothermo responsive polymer, Chem. Commun., 52, pp. 10521-10524, (2016)
  • [2] Hansen A., Zhang R., Bradley M., Et al., Fabrication of arrays of polymer gradients using inkjet printing, Macromol. Rapid Commun., 33, pp. 1114-1118, (2012)
  • [3] Yang F., Zhao X.Y., Wang X., Et al., Research progress in polymer fluorescent microspheres, Plastics Science and Technology, 41, 9, pp. 90-94, (2013)
  • [4] Liu Q.H., Liu J., Guo J.C., Et al., Preparation of polystyrene fluorescent microshperes based on some fluorescent labels, J. Mater. Chem., 19, pp. 2015-2018, (2009)
  • [5] Zhan R.Y., Tan A.J.H., Liu B., Conjugated polyelectrolyte as signal amplifier for fluorogenic probe based enzyme activity study, Polym. Chem., 2, pp. 417-421, (2011)
  • [6] Bao B.Q., Fan Q.L., Wang L.H., Et al., Progress in the study of conjugated polymer fluorescent nanoparticles, Scientia Sinica Chimica, 44, 3, pp. 282-294, (2014)
  • [7] Liu J.L., Mi H.Y., Guan M.M., Et al., Preparation and application of novel fluorescence chemical sensor for determining trace levels of picronitric acid based on water soluble conjugated polymer, Chemical Journal of Chinese University, 38, 12, pp. 2163-2168, (2017)
  • [8] Qian C.G., Zhu S., Feng P.J., Et al., Conjugated polymer nanoparticles for fluorescence imaging and sensing of neurotransmitter dopamine in living cells and the brains of zebrafish larvae, ACS Appl. Mater. Interfaces, 7, pp. 18581-18589, (2015)
  • [9] Chen B.B., Mi H., Gao S., Et al., Effect of substituted triphenylamines on photoluminescent properties of triphenylamine-fluorene-benzothiadiazole copolymers, Journal of Chemistry Engineering of Chinese Universities, 29, 4, pp. 1010-1015, (2015)
  • [10] Zhu C.L., Liu L.B., Yang Q., Et al., Water-soluble conjugated polymers for imaging, diagnosis, and therapy, Chem. Rev., 112, pp. 4687-4735, (2012)
12