Analysis of Schaftoside and It's Anti-inflammatory Activity in the Water Extracts from 8 Lotus Plumule

被引：0
|
作者：
Wang J. [1 ]
Li H. [1 ]
Pan S. [1 ]
Xu X. [1 ]
机构：
[1] Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan
来源：
Xu, Xiaoyun (xuxiaoyun@mail.hzau.edu.cn) | 2018年 / Chinese Institute of Food Science and Technology卷 / 18期
关键词：
Anti-inflammatory; Lotus plumule; Schaftoside;
D O I：
10.16429/j.1009-7848.2018.03.038
中图分类号：
学科分类号：
摘要：
The anti-inflammatory activity of water extract from 8 lotus plumule were studied in this paper. Firstly, the composition of flavones in water extract from lotus plumule (LPWE) was analysed by reversed-phase high performance liquid chromatogram (RP-HPLC). And schaftoside was the highest content with a percentage from 0.97% to 1.47% among all the flavones in the LPWEs. Secondly, RAW264.7 macrophages was stimulated by lipopolysaccharide to make an inflammatory model. And the anti-inflammatory activity of LPWE as well as schaftoside was evaluated. The correlation analysis between dose and activity was followed. The results showed that LPWE significantly reduced the expression of NO and the level of TNF-α. And the higher concentration of LPWE, the better anti-inflammatory activity was. What's more, the NO inhibition rate of 80 μg/mL LPWE was significantly (P<0.05) positive related with the content of schaftoside contained in it (R2=0.974). The research indicated that LPWE has a good anti-inflammatory activity, and schaftoside might be the key composition of LPWE's anti-inflammatory activity. Hence, choosing lotus plumule with a higher content of schaftoside might get a better anti-inflammatory activity. © 2018, Editorial Office of Journal of CIFST. All right reserved.
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页码：291 / 298
页数：7
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共 7 条
  • [1] Yang G., Li W., Pan Y., Rapid simultaneous determination of four Alkaloids in Lotus plumule by CZE with ephedrine hydrochloride as an internal standard, Chromatographia, 75, 21-22, pp. 1295-1300, (2012)
  • [2] Wang L., Yen J.H., Liang H.L., Antioxidant effect of methanol extracts from lotus plumule and blossom (Nelumbo nucifera Gertn.), Journal of Food and Drug Analysis, 11, 1, pp. 60-66, (2003)
  • [3] Lin J.Y., Lai Y.S., Liu C.J., Effects of lotus plumule supplementation before and following systemic administration of lipopolysaccharide on the splenocyte responses of BALB/c mice, Food and Chemical Toxicology, 45, 3, pp. 486-493, (2007)
  • [4] Lin J., Wu A., Liu C., Suppressive effects of lotus plumule (Nelumbo nucifera Geartn.) supplementation on LPS-induced systemic inflammation in a BALB/c mouse model, Journal of Food and Drug Analysis, 14, 3, pp. 273-280, (2006)
  • [5] Liao C.H., Lin J.Y., Purification, partial characterization and anti-inflammatory characteristics of lotus (Nelumbo nucifera Gaertn) plumule polysaccharides, Food Chemistry, 135, 3, pp. 1818-1827, (2012)
  • [6] Liao C.H., Lin J.Y., Purified active lotus plumule (Nelumbo nucifera Gaertn) polysaccharides exert anti-inflammatory activity through decreasing Toll-like receptor-2 and-4 expressions using mouse primary splenocytes, Journal of Ethnopharmacology, 147, 1, pp. 164-173, (2013)
  • [7] Chen S., Fang L., Xi H., Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus(Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry, Analytica Chimica Acta, 724, 4, pp. 127-135, (2012)
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