Studies on Preparation and Antioxidant Activity of Artemisia integrifolia Polysaccharides

Objective: Isolate and purify polysaccharides from Artemisia integrifolia and to investigate their antioxidant activity. Methods: The optimum extraction process was obtained by ultrasonic-assisted enzymatic extraction of polysaccharides from Artemisia integrifolia using response surface methodology. Results: The optimum extraction process was obtained as follows: ultrasonic power 300 W, material-liquid ratio 1:30 g/mL, ultrasonic time 46 min, addition amount of cellulase 2.0%, addition amount of pectinase 3.0%, and under this condition, polysaccharide yield 7.67%. A total of six polysaccharide fractions were obtained by separation and purification using DEAE-52 chromatography column. Antioxidant activity studies on both AIP-2 and AIP-3 fractions showed that both AIP-2 and AIP-3 treatments at different mass concentrations had strong concentration-dependent protective effects against H2O2-induced oxidative damage in FiepG2 cells. Compared with the model group, treatment with high mass concentrations of AIP-2 and AIP-3 (50 μg/mL) increased cell survival by 54.35% and 60.26%, SOD viability increased by 35.86% and 38.98%, CAT viability increased by 173.02% and 205.42%, GSH-PX viability increased by 50.24% and 50.78%, and MDA content decreased by 46.13% and 53.87%. Conclusion: Artemisia integrifolia polysaccharide has good antioxidant activity. © 2024 Chinese Institute of Food Science and Technology. All rights reserved.