Pharmacokinetics of Nine Effective Ingredients of Chaihuyujinxiang Granules in Rats with Depression by UPLC-MS/MS

被引：0
|
作者：
Jiang C.-F. [1 ]
Xue G.-H. [1 ]
Fu L.-J. [2 ]
Zhang J.-Q. [2 ]
Gao Y. [1 ]
Gao L. [1 ,2 ]
机构：
[1] College of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun
[2] Jilin Province Modern Chinese Medicine Engineering Research Center Co., Ltd., Changchun
来源：
Journal of Chinese Mass Spectrometry Society | 2022年 / 43卷 / 03期
关键词：
Bupleurum; Chaihuyujinxiang granules; Licorice; Pharmacokinetics; Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS);
D O I：
10.7538/zpxb.2021.0162
中图分类号：
学科分类号：
摘要：
Pharmacokinetics reflects the laws of medicine absorption, distribution, metabolism and excretion in the body. The multi-component pharmacokinetic study of traditional Chinese medicine (TCM) is a bridge between the chemical composition and pharmacodynamic activity, and it is the key to reveal the pharmacodynamic material basis of TCM and guide the rational use of clinical medicine. Therefore, it is very necessary to elucidate the pharmacokinetic characteristics of the main pharmacodynamic components of medicines in disease models. At present, the method of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has unique advantages in the analysis of complex TCM components and their metabolites in vivo, which is a powerful method for pharmacokinetic analysis. Chaihuyujinxiang Granules (CHYJX) is an evolution of the classic prescription Chaihu Shugan San, which is composed by Radix Bupleuri, Glycyrrhiza uralensis Fisch, Cyperus rotundus L, Radix Curcumae Aromaticae extracts and Ostrea gigas thunberg superfine powder. In this study, a method of UPLC-MS/MS was established for simultaneous quantification of saikosaponin a, saikosaponin c, saikosaponin d, glycyrrhetinic acid, liquiritin, isoliquiritigenin, liquiritigenin, formononetin, glycyrrhizic acid in rat plasma. The methodological investigation including selectivity, residue, linearity, precision and accuracy, extraction recovery rate and matrix effect, stability all met the requirements of quantitative analysis for biological samples. The rat model of depression was constructed by the method of chronic unpredictable mild stress (CUMS). The 24 h dynamic changes of the nine main pharmacodynamic components in the plasma of depressed rats after intragastric administration of CHYJX were studied. The contents of 9 components in rats showed regular changes over time. Peak time (Tmax), half-life (t1/2), area under the cure [AUC(0-t)], MRT(0-t) were calculated as 0.27-4.06 h, 3.51-7.76 h, 70.06-1 280.4 μg•h/L, 7.13-11.48 h, respectively. The CHYJX could be rapidly absorbed in the gastrointestinal tract, and the elimination speed was fast in the body, which showed that the medicine was not easy to produce residue and accumulation, and had satisfied safety requirements. According to the medicine-time curve results, it was found that saikosaponin a, saikosaponin c, saikosaponin d, isoliquiritigenin, liquiritigenin, and glycyrrhizic acid all had a second absorption peak, which indicated that the 6 components might undergo enterohepatic circulation, transformation of different compounds, multi-site absorption or intestinal efflux in vivo. The study not only provides a reference method for the simultaneous quantification of the 9 components in plasma, but also provides theoretical support for the basic research on pharmacodynamic substances and clinical application of CHYJX. © 2022, Editorial Board of Journal of Chinese Mass Spectrometry Society. All right reserved.
引用
收藏
页码：336 / 346
页数：10
相关论文
共 17 条
  • [1] HU Hui, Discussion on the etiology and pathogenesis of depression in traditional Chinese medicine, Shaanxi Traditional Chinese Medicine, 28, 2, (2007)
  • [2] SUN X P, LI X L, PAN R L, XU Y F, WANG Q, SONG M J., Total Saikosaponins of Bupleurum yinchowense reduces depressive, anxiety-like behavior and increases synaptic proteins expression in chronic corticosterine-treated mice, BMC Complement Altern Med, 18, 1, (2018)
  • [3] HE Zehui, FAN Rong, ZHANG Chunhu, TANG Tao, CUI Hanjin, LIU Xu, LIANG Qinghua, WANG Yang, Study on the regulation of cytochrome P450 enzyme by total saponins of Bupleurum to exert antidepressant effect similar to Chaihu Shugan Powder, Hunan Traditional Chinese Medicine Journal of the University, 39, 6, pp. 693-698, (2019)
  • [4] LIU Jingli, YAN Hao, WANG Yuying, WANG Wenliang, DFT study on the relationship between the structure and hepatoprotective activity of glycyrrhizin compounds, Natural Product Research and Development, 32, 9, pp. 1515-1521, (2020)
  • [5] ZONG Yang, HE Shufen, SUN Bingting, ZHANG Qian, JU Wenzheng, An overview of the mechanism of antidepressant action of licorice and its application, Chinese Journal of Experimental Formulas, 22, 10, pp. 194-198, (2016)
  • [6] KIM Y W, KI S H, LEE J R, LEE S J, KIM C W, KIM S C, KIM S G., Liquiritigenin, an aglycone of liquiritin in Glycyrrhizae radix, prevents acute liver injuries in rats induced by acetaminophen with or without buthionine sulfoximine, Chem Biol Interact, 161, 2, pp. 125-138, (2006)
  • [7] YANG Xiaoyu, SU Xiulan, Pathway mechanism of glycyrrhetic acid's hepatoprotective effect, Chinese Journal of Histochemistry and Cytochemistry, 28, 1, pp. 66-70, (2019)
  • [8] ZHAO Z L, PARK S M, GUAN L X, WU L X, LEE J R, KIM S C, KIM Y W, ZHAO R J., Isoliquiritigenin attenuates oxidative hepatic damage induced by carbon tetrachloride with or without buthionine sulfoximine, Chem Biol Interact, 225, pp. 13-20, (2015)
  • [9] ZHAO Z Y, WANG W X, GUO H Z, ZHOU D F., Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats, Behav Brain Res, 194, 1, pp. 108-113, (2008)
  • [10] QIU Z K, LIU Z T, PANG J L, WU H B, LIU X, YANG Z M, LI X, CHEN J S., A network pharmacology study with molecular docking to investigate the possibility of licorice against posttraumatic stress disorder, Metab Brain Dis, 36, 7, pp. 1763-1777, (2021)
12