Fabrication of Triphase Enzyme Electrode Based on Porous Gold Substrate for High-performance Electrochemical Biosensor

被引：0
|
作者：
Zhang J. [1 ]
Ding Z. [2 ]
Wang D. [1 ]
Chen L. [1 ]
Feng X. [1 ,2 ]
机构：
[1] College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou
[2] Innovation Center for Chemical Science, Soochow University, Suzhou
来源：
Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities | 2021年 / 42卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Enzymatic reaction; Enzyme biosensor; Microstructure; Triphase interface; Wettability;
D O I：
10.7503/cjcu20210355
中图分类号：
学科分类号：
摘要：
The interface microenvironment is the key factor affecting the enzymatic reaction and enzyme biosensing performance. In this study, we fabricated an oxidase enzyme electrode with an air-liquid-solid triphase interfacial microenvironment by adjusting the surface wettability of three-dimensional(3D) micro/nano- structured porous gold substrate, and studied the effects of interfacial microenvironment on enzymatic reaction kinetics and performance of enzyme electrode. Based on the triphase interface porous gold structured enzyme electrode, the reactant oxygen can be directly and quickly transferred from the gas phase to the enzyme reaction interface, which greatly increases the interface oxygen concentration and stability, thus enhances the oxidase activity and the stability of enzyme electrode response. Take glucose as a model analyte, we found that the electrochemical enzyme biosensor has a wide linear range, high sensitivity, low detection limit and good stability. This unique triphase reaction interface design strategy provides a new idea for the construction of high-performance enzyme biosensor and accurate detection of biomolecules. © 2021, Editorial Department of Chem. J. Chinese Universities. All right reserved.
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页码：3167 / 3174
页数：7
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