Distributions of Antibiotic Resistance Genes and Microbial Communities in the Nearshore Area of the Yangtze River Estuary

被引：0
|
作者：
Xu Q.-H. [1 ]
Liu S.-G. [1 ,2 ]
Lou S. [1 ,2 ]
Larisa D.R. [3 ]
Elena N. [3 ]
Makhinov A.N. [4 ]
Araruna J.T. [5 ]
Jin Y.-C. [1 ]
Li X. [1 ]
机构：
[1] Department of Hydraulic Engineering, Tongji University, Shanghai
[2] Key Laboratory of Yangtze River Water Environment, Ministry of Education, Shanghai
[3] Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of Siberian Branch, the Russian Academy of Sciences, Buryatia
[4] Institute of Water and Ecology Problems, Far East Branch, Russian Academy of Sciences, Khabarovsk
[5] Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro
来源：
Huanjing Kexue/Environmental Science | 2023年 / 44卷 / 01期
关键词：
16S amplicon sequencing; antibiotics resistance genes(ARGs); microbial communities; nearshore area of the Yangtze River Estuary; quantitative-polymerase chain reaction(qPCR);
D O I：
10.13227/j.hjkx.202203160
中图分类号：
学科分类号：
摘要：
Due to the extensive use of antibiotics, the resistance of microorganisms to antibiotics in the environment is increasing, and the problem of antibiotic resistance genes (ARGs) is becoming more and more severe, which seriously threatens ecological security and human health. In order to study the distribution characteristics of ARGs and the microbial community in different media in the coastal area of the Yangtze River Estuary, water and sediment samples from eight sites were collected through a field investigation. Two sulfonamide resistance genes (sul1, sul2) and six tetracycline resistance genes (tetM, tetC, tetX, tetA, tetO, and tetQ), one integrase gene intI1, 16S rRNA gene, and the microbial community were detected and analyzed. The results showed that the detection rate of 10 resistance genes in the coastal area of the Yangtze River Estuary was 100%. intI1 was significantly positively correlated with various ARGs in the water samples. Proteobacteria and Bacteroidota were the dominant bacteria phyla in the water environment of the Yangtze River Estuary. Chloeoplast was the main bacteria genus in water, and Chloeoplast and Niteospiea were the main bacteria genera in sediment. In water, Nitrospirota was the common potential host of four tetracycline resistance genes (tetX, tetA, tetO, and tetQ). In sediments, Sva0485 was a potential host community shared by sul1 and intI1. The distribution of the microbial community was an important factor affecting the migration and transformation of ARGs in the nearshore area of the Yangtze River Estuary. © 2023 Science Press. All rights reserved.
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页码：158 / 168
页数：10
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