Feed-additive of bioengineering strain with surface-displayed laccase degrades sulfadiazine in broiler manure and maintains intestinal flora structure

被引：0

作者：

Li, Rong ^{[1
,2
]}

Zhou, Tuoyu ^{[2
]}

Khan, Aman ^{[2
]}

Ling, Zhenmin ^{[2
]}

Sharma, Monika ^{[2
]}

Feng, Pengya ^{[2
]}

Ali, Gohar ^{[2
]}

Saif, Irfan ^{[2
]}

Wang, Haoyang ^{[3
]}

Li, Xiangkai ^{[2
]}

Liu, Pu ^{[1
]}

机构：

[1] Gansu Key Laboratory of Biomonitoring and Biorer mediation for Environment Pollution. School of Life Science, Lanzhou University, 222, South Tianshui rd, Lanzhou,Gansu,730000, China

[2] Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, No. 222, Tianshuinan Road, Lanzhou,Gansu,730000, China

[3] McMaster University, 303–2, 1100 Main Street West, Hamilton,ON, Canada

来源：

Journal of Hazardous Materials | 2021年 / 406卷

基金：

中国国家自然科学基金;

关键词：

Cell membranes - Fertilizers - Animals - Enzyme activity - Sulfur compounds - Antibiotics - Manures;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Sulfonamide antibiotics (SAs) are excreted into the ecosystem unchanged through feces and urine because of their low adsorption and degradation in the guts of humans and animals. In this study, a novel whole-cell biocatalyst with fungal laccase on the cell surface of Escherichia coli Nissle 1917 was developed to degrade sulfadiazine (SDZ). Engineered strain EcN-IL showed laccase enzyme activity of 2 ± 1 U/mg dry weight of cell and degraded 37 ± 1% of SDZ at temperature 40 °C and pH 5 within 3 h in vitro. Strain EcN-IL with 500 mg/kg of SDZ was employed as a food supplement to feed chicken broilers, which can reduce the residue of SDZ in broiler manure by 58 ± 2% and also reduced dysbiosis of the gut microbiota due to overuse of antibiotics. The genetically engineered EcN-IL has laid a foundation for degrading SDZ in broilers and their manure. © 2020 Elsevier B.V.

引用