Interaction between Stibnite and Microbial Communities Enriched from Tailings at Xikuangshan Coupling with Bacterial Community Succession

被引：0

作者：

Wang Y. ^{[1
]}

Wu M. ^{[1
]}

Wang W. ^{[1
]}

Li M. ^{[1
]}

Lei J. ^{[1
]}

Wang H. ^{[1
,2
]}

机构：

[1] School of Environmental Studies, China University of Geosciences, Wuhan

[2] State Key Laboratory of Geobiology and Environmental Geology, China University of Geosciences, Wuhan

来源：

Diqiu Kexue - Zhongguo Dizhi Daxue Xuebao/Earth Science - Journal of China University of Geosciences | 2023年 / 48卷 / 11期

关键词：

antimony oxidation; biogeology; environmental geology; geochemistry; interaction between microbe and mineral; microbial community succession; stibnite; tailing dam; Xikuangshan antimony deposit;

D O I：

10.3799/dqkx.2022.287

中图分类号：

学科分类号：

摘要：

To better understand microbial dissolution and oxidation of stibnite at the community level, microbial communities were enriched from the tailings in the Xikuangshan tailings pond, Hunan Province, which were further used to study microbial interaction with stibnite. pH, total Sb, Sb(V), Sb(III) and SO42- were measured periodically. 16S rRNA high-throughput sequencing and X-ray powder diffractometer (XRD) were exploited to detect compositions of microbial communities and mineral phases, respectively. The results show that microbial communities can grow with sodium lactate and increase the pH of the solution under aerobic condition, which facilitated the dissolution of stibnite. The concentration of Sb(V) accumulated in the solution due to the oxidation of the released Sb(III), with an decrease after the 12th day. The Sb(III) concentration in the solution increased after the 9th day and was a little bit higher than that of Sb(V) at the end of the experiments. The incomplete oxidation of Sb(III) may result from the low relative abundances of antimony-oxidizing bacteria such as Paracoccus and Bosea in the enriched culture. The variation of solution chemistry, particularly the Sb concentration, exerted strong selection on microbial communities. Microbial communities were dominated by Herbaspirillum sp., with strong resistance to metals, such as and Sb. However their relative abundances decreased after 11 days’ interaction potentially due to the accumulation of Sb in the system. Secondary Sb(V) -bearing mineral, sodium antimonite, was detected at the end of the experiments in the biotic systems, which matched well with the decrease of the concentration of Sb(V) in the solution. Our results further confirm the mechanism of microbial dissolution of stibnite followed by oxidation and transformation of minerals at the community level, which provides new insights of understanding the impact of microbial communities on the dissolution of Sb-bearing secondary minerals, oxidation, migration and formation of antimony in natural environments. © 2023 China University of Geosciences. All rights reserved.

引用

页码：4311 / 4320

页数：9

共 37 条

[1] Ali A., Li M., Su J., Et al., Brevundimonas diminu⁃ ta Isolated from Mines Polluted Soil Immobilized Cadmium (Cd2+ ) and Zinc (Zn2+ ) through Calcium Carbonate Precipitation: Microscopic and Spectroscopic Investigations, Science of Total Environment, 813, (2022)
[2] Biver M., Shotyk W., Stibnite (Sb2S3) Oxidative Dissolution Kinetics from pH 1 to 11, Geochimica et Cosmo⁃ chimica Acta, 79, pp. 127-139, (2012)
[3] Caporaso J. G., Kuczynski J., Stombaugh J., Et al., QIIME Allows Analysis of High ‐ Throughput Community Sequencing Data, Gut Microbes, 7, 5, pp. 335-336, (2010)
[4] Edgar R. C., UPARSE: Highly Accurate OTU Sequences from Microbial Amplicon Reads, Frontiers in Plant Science, 10, 10, pp. 996-998, (2013)
[5] Edgar R. C., Haas B. J., Clemente J. C., Et al., UCHIME Improves Sensitivity and Speed of Chimera Detection, Bioinformatics, 27, 16, pp. 2194-2200, (2011)
[6] Fang Q., Huang T., Wang N., Et al., Effects of Herbaspirillum sp. P5 ‐ 19 Assisted with Alien Soil Im ‐ provement on the Phytoremediation of Copper Tailings by Vetiveria zizanioides L, Environmental Science and Pollution Research, 28, 45, pp. 64757-64768, (2021)
[7] Fu Z., Wu F., Mo C., Et al., Comparison of Arsenic and Antimony Biogeochemical Behavior in Water, Soil and Tailings from Xikuangshan, China, Science of the Total Environment, 539, pp. 97-104, (2016)
[8] Govarthanan M., Lee G. W., Park J. H., Et al., Bioleaching Characteristics, Influencing Factors of Cu Solubilization and Survival of Herbaspirillum sp. GW103 in Cu Contaminated Mine Soil, Chemosphere, 109, pp. 42-48, (2014)
[9] Hamamura N., Fukushima K., Itai T, Identification of Antimony‐ and Arsenic‐Oxidizing Bacteria Associated with Antimony Mine Tailing, Microbes and Envi⁃ ronments, 28, 2, pp. 257-263, (2013)
[10] Hamamura N., Nakajima N., Yamamura S., Draft Genome Sequence of the Antimony‐Oxidizing Pseudomo⁃ nas sp. Strain SbOxS1, Isolated from Stibnite Mine Tailing Soil, Microbiology Resource Announcements, 9, 49, pp. e01218-e01220, (2020)

← 1 2 3 4 →